The regulatory landscape of the human HPF1- and ARH3-dependent ADP-ribosylome

Ivo A. Hendriks; Sara C. Buch-Larsen; Evgeniia Prokhorova; Jonas D. Elsborg; Alexandra K.L.F.S. Rebak; Kang Zhu; Dragana Ahel; Claudia Lukas; Ivan Ahel; Michael L. Nielsen

doi:10.1038/s41467-021-26172-4

Nature Communications (Oct 2021)

The regulatory landscape of the human HPF1- and ARH3-dependent ADP-ribosylome

Ivo A. Hendriks,
Sara C. Buch-Larsen,
Evgeniia Prokhorova,
Jonas D. Elsborg,
Alexandra K.L.F.S. Rebak,
Kang Zhu,
Dragana Ahel,
Claudia Lukas,
Ivan Ahel,
Michael L. Nielsen

Affiliations

Ivo A. Hendriks: Proteomics Program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen
Sara C. Buch-Larsen: Proteomics Program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen
Evgeniia Prokhorova: Sir William Dunn School of Pathology, University of Oxford
Jonas D. Elsborg: Proteomics Program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen
Alexandra K.L.F.S. Rebak: Proteomics Program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen
Kang Zhu: Sir William Dunn School of Pathology, University of Oxford
Dragana Ahel: Sir William Dunn School of Pathology, University of Oxford
Claudia Lukas: Protein Signaling Program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen
Ivan Ahel: Sir William Dunn School of Pathology, University of Oxford
Michael L. Nielsen: Proteomics Program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen

DOI: https://doi.org/10.1038/s41467-021-26172-4
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 16

Abstract

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ADP-ribosylation is regulated by HPF1 and ARH3, but the cellular target spectrum of these enzymes is not fully understood. Here, the authors use quantitative proteomics to define the HPF1- and ARH3-dependent ADP-ribosylome, providing evidence that mono-ADP-ribosylation of serine predominates in cells.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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