Standards in Genomic Sciences (Dec 2017)

Draft genome sequence of Bacillus velezensis 2A-2B strain: a rhizospheric inhabitant of Sporobolus airoides (Torr.) Torr., with antifungal activity against root rot causing phytopathogens

  • Inés Martínez-Raudales,
  • Yumiko De La Cruz-Rodríguez,
  • Alejandro Alvarado-Gutiérrez,
  • Julio Vega-Arreguín,
  • Ahuitz Fraire-Mayorga,
  • Miguel Alvarado-Rodríguez,
  • Victor Balderas-Hernández,
  • Saúl Fraire-Velázquez

DOI
https://doi.org/10.1186/s40793-017-0289-4
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 10

Abstract

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Abstract A Bacillus velezensis strain from the rhizosphere of Sporobolus airoides (Torr.) Torr., a grass in central-north México, was isolated during a biocontrol of phytopathogens scrutiny study. The 2A-2B strain exhibited at least 60% of growth inhibition of virulent isolates of phytopathogens causing root rot. These phytopathogens include Phytophthora capsici, Fusarium solani, Fusarium oxysporum and Rhizoctonia solani. Furthermore, the 2A-2B strain is an indolacetic acid producer, and a plant inducer of PR1, which is an induced systemic resistance related gene in chili pepper plantlets. Whole genome sequencing was performed to generate a draft genome assembly of 3.953 MB with 46.36% of GC content, and a N50 of 294,737. The genome contains 3713 protein coding genes and 89 RNA genes. Moreover, comparative genome analysis revealed that the 2A-2B strain had the greatest identity (98.4%) with Bacillus velezensis.

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