Chinese Journal of Lung Cancer (Apr 2010)
Biological Characteristics and Antitumor Activity of CIK Cells Activated by Recombinant Human Fibronectin for Human Lung Cancer Cell Lines In Vitro
Abstract
Background and objective The CIK cell is one of the most important means of the adoptive cellular immunotherapy, and it is a hotspot of which to simplify its culture procedure and to promote its inhibition rate. The aim of this study is to observe the biological function of the CIK cells cultivated by the recombinant human fibronectin (RN) and to establish an effective and simple way of cells expansion. Methods We separated the mononuclear cells (PBMCs) in 50 mL peripheral blood from 10 healthy persons with density gradient centrifugation in the lymphocyte-separating medium, and the PBMCs were divided into two groups, of which were cultivated by RN-introduced and conventional method separately. Then we estimated the proliferation ability, and analyzed the immunologic type, IFN-γ, IL-4, perforin and granzyme B of them with flow cytometry. Besides that, we tested the inhibition rate of CIKs cells to four kinds of human lung cancer cell lines in vitro by MTT assay. Results The RN-induced group had a higher proliferation rate that was 2.0-3.5 times of the conventional group, and there was an obvious statistical difference between the two (P 0.05). We found a similar inhibition rate of the CIK cells to all this human lung cancer cell lines (P >0.05). The cells which secreted IFN–γ increased, while the cells which secreted IL-4 did not. The cells which secreted granzyme B and perforin were positive. Conclusion It is an effective and simple way to cultivate the CIK cells with RN, which should be adopted.
