STAR Protocols (Dec 2022)

Protocol to desensitize human and murine mast cells after polyclonal IgE sensitization

  • Celia López-Sanz,
  • Elisa Sánchez-Martínez,
  • Rodrigo Jiménez-Saiz

Journal volume & issue
Vol. 3, no. 4
p. 101755

Abstract

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Summary: In this protocol, we provide detailed instructions to desensitize human and murine mast cells (MCs) after polyclonal IgE sensitization. Moreover, we specify the steps for MC degranulation assessment after desensitization, measuring CD63 and CD107a expression by flow cytometry and β-hexosaminidase activity. Desensitized MCs can be used directly for co-culture with other cell types, immunofluorescence, live imaging, and omics approaches.For complete details on the use and execution of this protocol, please refer to López-Sanz et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

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