Genetic diversity of durum wheat as determined by AFLP in fluorescence

F. Sciacca; C. Fichera; S. Di Silvestro; E. Conte; M. Palumbo

doi:10.1007/s10535-010-0035-x

Biologia Plantarum (Mar 2010)

Genetic diversity of durum wheat as determined by AFLP in fluorescence

F. Sciacca,
C. Fichera,
S. Di Silvestro,
E. Conte,
M. Palumbo

Affiliations

F. Sciacca: Agricultural Research Council, Mediterranean Crops Research Center, Acireale, Italy
C. Fichera: Agricultural Research Council, Mediterranean Crops Research Center, Acireale, Italy
S. Di Silvestro: Science and Technology Park of Sicily, Zona Industriale Blocco Palma I, Catania, Italy
E. Conte: Science and Technology Park of Sicily, Zona Industriale Blocco Palma I, Catania, Italy
M. Palumbo: Agricultural Research Council, Mediterranean Crops Research Center, Acireale, Italy

DOI: https://doi.org/10.1007/s10535-010-0035-x
Journal volume & issue: Vol. 54, no. 1
pp. 198 – 200

Abstract

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The DNA of fifteen Italian cultivars of durum wheat (Triticum turgidum L. ssp. durum) were analyzed by in fluorescence amplified fragment length polymorphism (fAFLP) in order to obtain the characteristic fingerprintings of genotypes and assess their genetic relatedness. Among 64 combinations of fluorescence labelled primers, three different combinations were chosen as producing a total of 6630 AFLP fragments, 2277 (34.3 %) of them being polymorphic. By using this fAFLP methodology a DNA fingerprinting of each durum wheat cultivar was generated for genotype identification. Analysis of the genetic relationships show the low variability among durum wheat cultivars.

Published in Biologia Plantarum

ISSN: 1573-8264 (Online)
Publisher: Institute of Experimental Botany of the Czech Academy of Sciences
Country of publisher: Czechia
LCC subjects: Science: Biology (General); Science: Botany: Plant ecology
Website: https://bp.ueb.cas.cz/

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