Design and building of modern two-photon laser scanning microscope for using in neuroscience

Abstract

One of the main visualization method in neuroscience is two-photon microscopy. However, scientists need to upgrade their microscopy system so regular because they are interested to get more specific data. Also this is due to the fact that this technique allows to obtain 3D images of tissues due to laser focus change, that is possible due to substantially greater penetration depth on the main wavelength into biological tissues. Self-developed microscopy system allow to modify the construction of microscope in not-complicated manner depending on specialized experimental models and scientific tasks. This work is small attempt to help researchers who are interested to build their own two-photon laser scanning microscope for using in neuroscience. The work was performed with financial support of the government represented by the Ministry of Education and Science of the Russian Federation, the unique identifier of the project is RFMEFI57814X0079, the agreement on granting a subsidy №14.578.21.0079 dated 28.11.2014.