Frontiers in Bioengineering and Biotechnology (Mar 2022)

High Sensitivity, Rapid Detection of Virus in High Traffic Environments

  • Lauren Waller,
  • Zhilin Guo,
  • Rui Tang,
  • Zunming Zhang,
  • Edward Wang,
  • Jarred Yasuhara-Bell,
  • Louise Laurent,
  • Yu-Hwa Lo

DOI
https://doi.org/10.3389/fbioe.2022.877603
Journal volume & issue
Vol. 10

Abstract

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The global pandemic caused by the SARS-CoV-2 virus has underscored the need for rapid, simple, scalable, and high-throughput multiplex diagnostics in non-laboratory settings. Here we demonstrate a multiplex reverse-transcription loop-mediated isothermal amplification (RT-LAMP) coupled with a gold nanoparticle-based lateral flow immunoassay (LFIA) capable of detecting up to three unique viral gene targets in 15 min. RT-LAMP primers associated with three separate gene targets from the SARS-CoV-2 virus (Orf1ab, Envelope, and Nucleocapsid) were added to a one-pot mix. A colorimetric change from red to yellow occurs in the presence of a positive sample. Positive samples are run through a LFIA to achieve specificity on a multiplex three-test line paper assay. Positive results are indicated by a characteristic crimson line. The device is almost fully automated and is deployable in any community setting with a power source.

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