Prognostic significance of serum Chemerin and neutrophils levels in patients with oral squamous cell carcinoma
Xiaoyuan Hu,
Ning Wang,
Fei Gao,
Shengyou Ge,
Mei Lin,
Xuan Zhang,
Tongtong Li,
Tao Li,
Changting Xu,
Caixiu Huang,
Guicai Liang,
Wei Shang,
Fenggang Xiang,
Yuanyong Feng
Affiliations
Xiaoyuan Hu
Biological Therapy Center, The Third Affiliated Hospital of Kunming Medical University, Kunzhou Road No. 519, Kunming, Yunnan, 650118, China
Ning Wang
Department of Pathology, School of Basic Medicine, Qingdao University, Qingdao, 266003, China
Fei Gao
Deparment of Pathology, West China Hospital, Sichuan University, Sichuan, 610041, China
Shengyou Ge
Department of Oral and Maxillofacial Surgery, School of Stomatology and The Affiliated Hospital of Qingdao University, Jiangsu Road No.16, Qingdao, Shandong, 266003, China
Mei Lin
Department of Pathology, School of Basic Medicine, Qingdao University, Qingdao, 266003, China
Xuan Zhang
Department of Pathology, School of Basic Medicine, Qingdao University, Qingdao, 266003, China
Tongtong Li
Department of Pathology, School of Basic Medicine, Qingdao University, Qingdao, 266003, China
Tao Li
Biological Therapy Center, The Third Affiliated Hospital of Kunming Medical University, Kunzhou Road No. 519, Kunming, Yunnan, 650118, China
Changting Xu
Biological Therapy Center, The Third Affiliated Hospital of Kunming Medical University, Kunzhou Road No. 519, Kunming, Yunnan, 650118, China
Caixiu Huang
Biological Therapy Center, The Third Affiliated Hospital of Kunming Medical University, Kunzhou Road No. 519, Kunming, Yunnan, 650118, China
Guicai Liang
Biological Therapy Center, The Third Affiliated Hospital of Kunming Medical University, Kunzhou Road No. 519, Kunming, Yunnan, 650118, China
Wei Shang
Department of Oral and Maxillofacial Surgery, School of Stomatology and The Affiliated Hospital of Qingdao University, Jiangsu Road No.16, Qingdao, Shandong, 266003, China
Fenggang Xiang
Department of Pathology, School of Basic Medicine, Qingdao University, Qingdao, 266003, China; Corresponding author.
Yuanyong Feng
Department of Oral and Maxillofacial Surgery, School of Stomatology and The Affiliated Hospital of Qingdao University, Jiangsu Road No.16, Qingdao, Shandong, 266003, China; Corresponding author.
Objectives: Chemerin, as a novel multifunctional adipokine, is proposed to be involved in high cancer risk and mortality. The present study was aimed to evaluate the prognostic value of serum Chemerin and neutrophils in patients with oral squamous cell carcinoma (OSCC). Materials and methods: 120 patients with OSCC were included in this prospective cohort study. The levels of serum Chemerin were measured by enzyme-linked immunosorbent assay (ELISA). We also explored the possible effects of Chemerin on neutrophils’ chemokines in OSCC using a real-time PCR, western blotting. Results: Levels of serum Chemerin, neutrophils and NLR were significantly higher among non-survivors compared to survivors of OSCC (both P < 0.05). Higher serum Chemerin levels were associated with advanced TNM stage, lymph node metastasis, differentiation and tumor recurrence (both P < 0.05). Serum Chemerin levels correlated with neutrophils and NLR levels (r = 0.708, r = 0.578, both P < 0.05). Based on ROC analysis, Chemerin + NLR predicted OSCC patient mortality with 81.54 % sensitivity and 87.27 % specificity, with an AUC of 0.8898. In a Kaplan-Meier analysis, high serum Chemerin levels, high neutrophil levels and high NLR levels were associated with shorter overall and disease-free survival (both P < 0.05). A univariate and multivariate Cox regression analysis showed that serum Chemerin and neutrophils were independent risk factors for OSCC. (both P < 0.05). QRT-PCR and western blotting results showed that Chemerin upregulated the expression of chemokines IL-17 and CXCL-5 in neutrophils (both P < 0.05). Conclusions: Our study suggests that measurement of serum Chemerin and neutrophils might be a useful diagnostic and prognostic biomarker for OSCC patients. Chemerin may promote neutrophils infiltration in OSCC through upregulation of chemokines IL17 and CXCL-5.
