Genetic aberrations in iPSCs are introduced by a transient G1/S cell cycle checkpoint deficiency

Ryoko Araki; Yuko Hoki; Tomo Suga; Chizuka Obara; Misato Sunayama; Kaori Imadome; Mayumi Fujita; Satoshi Kamimura; Miki Nakamura; Sayaka Wakayama; Andras Nagy; Teruhiko Wakayama; Masumi Abe

doi:10.1038/s41467-019-13830-x

Nature Communications (Jan 2020)

Genetic aberrations in iPSCs are introduced by a transient G1/S cell cycle checkpoint deficiency

Ryoko Araki,
Yuko Hoki,
Tomo Suga,
Chizuka Obara,
Misato Sunayama,
Kaori Imadome,
Mayumi Fujita,
Satoshi Kamimura,
Miki Nakamura,
Sayaka Wakayama,
Andras Nagy,
Teruhiko Wakayama,
Masumi Abe

Affiliations

Ryoko Araki: Department of Basic Medical Sciences for Radiation Damages, National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology
Yuko Hoki: Department of Basic Medical Sciences for Radiation Damages, National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology
Tomo Suga: Department of Basic Medical Sciences for Radiation Damages, National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology
Chizuka Obara: Department of Basic Medical Sciences for Radiation Damages, National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology
Misato Sunayama: Department of Basic Medical Sciences for Radiation Damages, National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology
Kaori Imadome: Department of Basic Medical Sciences for Radiation Damages, National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology
Mayumi Fujita: Department of Basic Medical Sciences for Radiation Damages, National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology
Satoshi Kamimura: Department of Basic Medical Sciences for Radiation Damages, National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology
Miki Nakamura: Department of Basic Medical Sciences for Radiation Damages, National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology
Sayaka Wakayama: Advanced Biotechnology Center, University of Yamanashi
Andras Nagy: Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital
Teruhiko Wakayama: Advanced Biotechnology Center, University of Yamanashi
Masumi Abe: Department of Basic Medical Sciences for Radiation Damages, National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology

DOI: https://doi.org/10.1038/s41467-019-13830-x
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 17

Abstract

Read online

Point mutations have been found in induced pluripotent stem cells (iPSCs) but when they arise is unclear. Here, the authors show that a G1/S cell cycle checkpoint deficiency transiently occurs early in genome reprogramming, suggesting a common developmental pathway between iPSC and tumorigenesis, and generate genetic burden-free human iPSCs.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal