Vertical flow immunoassay for multiplex mycotoxins based on photonic nitrocellulose and SERS nanotags

Ruipeng Chen; Jingfang Hu; Hui Wang; Cheng Li; Haiqi Kang; Yuxuan Chen; Liang Yang; Xiangfang Tang; Benhai Xiong; Xiangwei Zhao

Food Chemistry: X (Jan 2025)

Vertical flow immunoassay for multiplex mycotoxins based on photonic nitrocellulose and SERS nanotags

Ruipeng Chen,
Jingfang Hu,
Hui Wang,
Cheng Li,
Haiqi Kang,
Yuxuan Chen,
Liang Yang,
Xiangfang Tang,
Benhai Xiong,
Xiangwei Zhao

Affiliations

Ruipeng Chen: State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China
Jingfang Hu: Beijing key Laboratory of Sensor, Beijing Information Science & Technology University, Beijing 100101, China
Hui Wang: State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China
Cheng Li: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China
Haiqi Kang: College of Economics and Management, Beijing University of Agriculture, Beijing 102206, China
Yuxuan Chen: State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China
Liang Yang: State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China
Xiangfang Tang: State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; Corresponding authors.
Benhai Xiong: State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; Corresponding authors.
Xiangwei Zhao: State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China; Southeast University Shenzhen Research Institute, Shenzhen 518000, China; Corresponding author at: State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.

Journal volume & issue: Vol. 25
p. 102152

Abstract

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Here, we report a SERS based VFA using PNC as a sensing substrate for highly sensitive multiplex mycotoxins detection. The PNC was fabricated by filtration-based self-assembled monodisperse SiO2 NPs on a filter membrane as a template, and the obtained PNC had an ordered complementary inverse opal structure. In parallel, three kinds of Raman dyes encoding Au@NBAAg, Au@4-MBAAg and Au@DNTBAg SERS nanotags were synthesized for the detection of OTA, AFB1 and ZON. In the immunoassay, because of the slow-photon effect of the PNC substrate, which facilitates the light coupling with SERS nanotags, the SERS signal was highly enhanced. The LODs of 8.2, 13.7 and 47.6 fg mL−1 were achieved for simultaneous detection of OTA, AFB1 and ZON, respectively, which were lower than the tolerable cutoff values recommended by the EC. Therefore, this proposed PNC based SERS VFA is expected to be a powerful method for multiplex mycotoxins detection.

Published in Food Chemistry: X

ISSN: 2590-1575 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply; Technology: Chemical technology: Food processing and manufacture
Website: https://www.journals.elsevier.com/food-chemistry-x

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