A New Biorecognition-Element-Free IDμE Sensor for the Identification and Quantification of <i>E. coli</i>
Yung-Kai Lin,
Hsing-Ju Wu,
Nguyen Van Hieu,
Pei-Yi Chu,
Thi Vien Thao Do,
Fiona Yan-Dong Yao,
Thien Luan Phan,
Congo Tak Shing Ching
Affiliations
Yung-Kai Lin
Institute of Food Safety and Risk Management, National Taiwan Ocean University, Keelung City 202, Taiwan
Hsing-Ju Wu
Research Assistant Center, Show Chwan Memorial Hospital, Changhua City 500, Taiwan
Nguyen Van Hieu
Department of Physics and Electronic Engineering, University of Science (Vietnam National University of Ho Chi Minh City), Ho Chi Minh City 700000, Vietnam
Pei-Yi Chu
Department of Pathology, Show Chwan Memorial Hospital, Changhua City 500, Taiwan
Thi Vien Thao Do
Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung City 402, Taiwan
Fiona Yan-Dong Yao
Division of Science, Engineering and Health Studies, The Hong Kong Polytechnic University-Hong Kong Community College, Hong Kong
Thien Luan Phan
Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung City 402, Taiwan
Congo Tak Shing Ching
Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung City 402, Taiwan
The label-free biosensor has emerged as an effective tool for the purpose of early detection of causative pathogens such as Escherichia coli as a preventive measure. In this study, a biorecognition-element-free interdigitated microelectrode (IDμE) sensor is designed and developed with this in mind, with good reliability and affordability. Results show that the designed sensor can identify E. coli with good selectivity using an impedance and capacitance of 7.69 MHz. At its optimum impedance of 1.3 kHz, the IDμE sensor can reliably quantify E. coli in a range of measurement (103.2~106 cfu/mL), linearity (R2 = 0.97), sensitivity (18.15 kΩ/log (cfu/mL)), and limit of detection (103.2 cfu/mL). In summary, the IDμE sensor developed possesses high potential for industrial and clinical applications.
