Alcohol Dehydrogenase of a Novel Algae Fermentation Strain Meyerozyma guilliermondii

Abstract

The production of bioethanol from algae has attracted wide attention in the field of energy. A novel algae fermentation strain, Meyerozyma guilliermondii, can produce alginate mlyase and alcohol dehydrogenase (ADH) at the same time. At present, there is no research on the fermentation conditions, separation, and purification of the alcohol dehydrogenase of Meyerozyma guilliermondii. In this research, the fermentation conditions of Meyerozyma guilliermondii, the separation, and purification processes of alcohol dehydrogenase, and the performance study of alcohol dehydrogenase were studied. According to the experimental results, the optimum fermentation conditions for enzyme production were as follows: fermentation medium with alginate, initial pH 5.0, and fermentation temperature of 30 °C. The highest enzyme activity reached 69.9 U mL–1 crude enzyme. The optimal treating time of the ultrasonic separation procedure was 12 min at power of 320 W. The suitable purification method of the enzyme was salting out and dialysis method. When saturation reached 50 %, the total enzyme activity was the highest. Finally, the properties of enzyme were studied in this research. The maximum activity was reached at pH 7. The optimum temperature of ADH activity was 35 °C.

Keywords

• algae fermentation strains
• ethanol production
• alcohol dehydrogenase