Journal of Education, Health and Sport (Nov 2015)
Разработка модели экспериментального пародонтита у крыс в условиях действия антагониста витамина К = The development of the experimental model of periodontitis of rats in conditions of the action of the antagonist of vitamin K
Abstract
Nikolaeva A. V. Разработка модели экспериментального пародонтита у крыс в условиях действия антагониста витамина К = The development of the experimental model of periodontitis of rats in conditions of the action of the antagonist of vitamin K. Journal of Education, Health and Sport. 2015;5(11):151-158. ISSN 2391-8306. DOIhttp://dx.doi.org/10.5281/zenodo.33658 http://ojs.ukw.edu.pl/index.php/johs/article/view/2015%3B5%2811%29%3A151-158 https://pbn.nauka.gov.pl/works/668269 Formerly Journal of Health Sciences. ISSN 1429-9623 / 2300-665X. Archives 2011–2014http://journal.rsw.edu.pl/index.php/JHS/issue/archive Deklaracja. Specyfika i zawartość merytoryczna czasopisma nie ulega zmianie. Zgodnie z informacją MNiSW z dnia 2 czerwca 2014 r., że w roku 2014 nie będzie przeprowadzana ocena czasopism naukowych; czasopismo o zmienionym tytule otrzymuje tyle samo punktów co na wykazie czasopism naukowych z dnia 31 grudnia 2014 r. The journal has had 5 points in Ministry of Science and Higher Education of Poland parametric evaluation. Part B item 1089. (31.12.2014). © The Author (s) 2015; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland and Radom University in Radom, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 05.09.2015. Revised 05.10.2015. Accepted: 10.11.2015. УДК: [616.314.7-008.1-02(086.48)+615.015.23+577.16]:599.323.4 UDC: [616.314.7-008.1-02(086.48)+615.015.23+577.16]:599.323.4 РАЗРАБОТКА МОДЕЛИ ЭКСПЕРИМЕНТАЛЬНОГО ПАРОДОНТИТА У КРЫС В УСЛОВИЯХ ДЕЙСТВИЯ АНТАГОНИСТА ВИТАМИНА К THE DEVELOPMENT OF THE EXPERIMENTAL MODEL OF PERIODONTITIS OF RATS IN CONDITIONS OF THE ACTION OF THE ANTAGONIST OF VITAMIN K А. В. Николаева A.V. Nikolaeva Государственное учреждение «Институт стоматологии Национальной Академии Медицинских Наук Украины» SE «The Institute of Stomatology National Academy of Medical Sciences of Ukraine» Реферат В опытах на 14 белых крысах-самцах была воспроизведена экспериментальная модель пародонтита с помощью варфарина. Под действием варфарина разрушался коллаген десны, снижалось содержание гликозаминогликанов в кости альвеолярного отростка, увеличивался уровень содержания сиаловых кислот в сыворотке крови крыс. Антагонист витамина К вызывал усиление резорбции костных структур пародонта, в слизистой оболочке полости рта увеличивалась активность провоспалительного фермента – кислой фосфатазы, а также содержание МДА в кости альвеолярного отростка. В соединительной ткани собственной пластинки СОПР отмечалось наличие умеренно выраженных реактивных изменений под влиянием варфарина. Ключевые слова: варфарин, антагонист витамина К, межклеточный матрикс, коллаген, гликозаминогликаны, провоспалительный фермент, резорбция костной ткани пародонта, белые крысы. A.V. Nikolaeva SE «The Institute of Stomatology of National Academy of Medical Sciences of Ukraine» THE DEVELOPMENT OF THE EXPERIMENTAL MODEL OF PERIODONTITIS OF RATS IN CONDITIONS OF THE ACTION OF THE ANTAGONIST OF VITAMIN K The Aim of the study was the development of experimental model of the periodontitis at rats, similar to the human periodontitis by using the antagonist of the vitamin K - warfarin. Materials and methods. The experiment was conducted on 14 white male rats (2 groups), which were kept on a standard diet of the vivarium. The first group of rats was intact (6 animals). In the second group was simulated an experimental parodontitis in 8 rats that received the antagonist of the vitamin K - warfarin - the anticoagulant of indirect action in a dose of 1 mg / kg body weight of rats. The experiment lasted 50 days. Results and conclusions. Under the action of warfarin began the degradation of the MKM of periodontal, as evidenced by: the destruction of collagen of gum, downward trend of the GAG of periodontal bone structure, increasing the level of sialic acids in the blood serum. Antagonist caused an increased of resorption of alveolar bone; increased the activity of proinflammatory enzyme - acid phosphatasein in the gums and oral mucosa, and the MDA content in the alveolar bone. At the connective tissue of the lamina propria of the oral mucosa was noted the presence of moderately severe reactive changes under the influence of the antagonist of the vitamin K. Key words: warfarin, vitamin K antagonist, extracellular matrix, collagen, glycosaminoglycans, a proinflammatory enzyme, periodontal bone resorption, white rats.
