Nature Communications (Dec 2020)

Revealing the structures of megadalton-scale DNA complexes with nucleotide resolution

  • Massimo Kube,
  • Fabian Kohler,
  • Elija Feigl,
  • Baki Nagel-Yüksel,
  • Elena M. Willner,
  • Jonas J. Funke,
  • Thomas Gerling,
  • Pierre Stömmer,
  • Maximilian N. Honemann,
  • Thomas G. Martin,
  • Sjors H. W. Scheres,
  • Hendrik Dietz

DOI
https://doi.org/10.1038/s41467-020-20020-7
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 10

Abstract

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Precision design of DNA origami needs precision validation. Here, the authors developed cryo-EM methods for obtaining high resolution structural data and for constructing pseudo-atomic models in a semi-automated fashion, allowing for iterative nanodevice inspection and refinement.