Nature Communications (Apr 2020)
Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues
- Etsuo A. Susaki,
- Chika Shimizu,
- Akihiro Kuno,
- Kazuki Tainaka,
- Xiang Li,
- Kengo Nishi,
- Ken Morishima,
- Hiroaki Ono,
- Koji L. Ode,
- Yuki Saeki,
- Kazunari Miyamichi,
- Kaoru Isa,
- Chihiro Yokoyama,
- Hiroki Kitaura,
- Masako Ikemura,
- Tetsuo Ushiku,
- Yoshihiro Shimizu,
- Takashi Saito,
- Takaomi C. Saido,
- Masashi Fukayama,
- Hirotaka Onoe,
- Kazushige Touhara,
- Tadashi Isa,
- Akiyoshi Kakita,
- Mitsuhiro Shibayama,
- Hiroki R. Ueda
Affiliations
- Etsuo A. Susaki
- Department of Systems Pharmacology, Graduate School of Medicine, The University of Tokyo
- Chika Shimizu
- Laboratory for Synthetic Biology, RIKEN Center for Biosystems Dynamics Research
- Akihiro Kuno
- Department of Systems Pharmacology, Graduate School of Medicine, The University of Tokyo
- Kazuki Tainaka
- Department of System Pathology for Neurological Disorders, Brain Research Institute, Niigata University
- Xiang Li
- Neutron Science Laboratory, The Institute for Solid State Physics, The University of Tokyo
- Kengo Nishi
- Neutron Science Laboratory, The Institute for Solid State Physics, The University of Tokyo
- Ken Morishima
- Neutron Science Laboratory, The Institute for Solid State Physics, The University of Tokyo
- Hiroaki Ono
- Department of Systems Pharmacology, Graduate School of Medicine, The University of Tokyo
- Koji L. Ode
- Department of Systems Pharmacology, Graduate School of Medicine, The University of Tokyo
- Yuki Saeki
- Faculty of Medicine, The University of Tokyo
- Kazunari Miyamichi
- Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
- Kaoru Isa
- Department of Neuroscience, Graduate School of Medicine and Faculty of Medicine, Kyoto University, Yoshida-konoe-cho
- Chihiro Yokoyama
- Laboratory for Brain Connectomics Imaging, RIKEN Center for Biosystems Dynamics Research, 6-7-3 Minatojima-minamimachi, Chuo-ku
- Hiroki Kitaura
- Department of Pathology, Brain Research Institute, Niigata University
- Masako Ikemura
- Department of Pathology, Graduate School of Medicine, The University of Tokyo
- Tetsuo Ushiku
- Department of Pathology, Graduate School of Medicine, The University of Tokyo
- Yoshihiro Shimizu
- Laboratory for Cell-Free Protein Synthesis, RIKEN Center for Biosystems Dynamics Research, 6-2-3, Furuedai
- Takashi Saito
- Laboratory for Proteolytic Neuroscience, RIKEN Center for Brain Science
- Takaomi C. Saido
- Laboratory for Proteolytic Neuroscience, RIKEN Center for Brain Science
- Masashi Fukayama
- Department of Pathology, Graduate School of Medicine, The University of Tokyo
- Hirotaka Onoe
- Human Brain Research Center, Graduate School of Medicine, Kyoto University
- Kazushige Touhara
- Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
- Tadashi Isa
- Department of Neuroscience, Graduate School of Medicine and Faculty of Medicine, Kyoto University, Yoshida-konoe-cho
- Akiyoshi Kakita
- Department of Pathology, Brain Research Institute, Niigata University
- Mitsuhiro Shibayama
- Neutron Science Laboratory, The Institute for Solid State Physics, The University of Tokyo
- Hiroki R. Ueda
- Department of Systems Pharmacology, Graduate School of Medicine, The University of Tokyo
- DOI
- https://doi.org/10.1038/s41467-020-15906-5
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 22
Abstract
Tissue clearing has revolutionised histology, but limited penetration of antibodies and stains into thick tissue segments is still a bottleneck. Here, the authors characterise optically cleared tissue as an electrolyte gel and apply this knowledge to stain the entirety of thick tissue samples.
