Mechanical, Structural, and Biological Characteristics of Polylactide/Wollastonite 3D Printed Scaffolds
Rajan Choudhary,
Inna Bulygina,
Vladislav Lvov,
Anna Zimina,
Sergey Zhirnov,
Evgeny Kolesnikov,
Denis Leybo,
Natalya Anisimova,
Mikhail Kiselevskiy,
Maria Kirsanova,
Fedor Senatov
Affiliations
Rajan Choudhary
Rudolfs Cimdins Riga Biomaterials Innovations and Development Centre of RTU, Faculty of Materials Science and Applied Chemistry, Institute of General Chemical Engineering, Riga Technical University, Kipsala Street 6A, LV-1048 Riga, Latvia
Inna Bulygina
Center for Biomedical Engineering, National University of Science and Technology “MISIS”, Leninskiy pr., 6s7, 119049 Moscow, Russia
Vladislav Lvov
Center for Biomedical Engineering, National University of Science and Technology “MISIS”, Leninskiy pr., 6s7, 119049 Moscow, Russia
Anna Zimina
Center for Biomedical Engineering, National University of Science and Technology “MISIS”, Leninskiy pr., 6s7, 119049 Moscow, Russia
Sergey Zhirnov
Center for Biomedical Engineering, National University of Science and Technology “MISIS”, Leninskiy pr., 6s7, 119049 Moscow, Russia
Evgeny Kolesnikov
Center for Biomedical Engineering, National University of Science and Technology “MISIS”, Leninskiy pr., 6s7, 119049 Moscow, Russia
Denis Leybo
Center for Biomedical Engineering, National University of Science and Technology “MISIS”, Leninskiy pr., 6s7, 119049 Moscow, Russia
Natalya Anisimova
Center for Biomedical Engineering, National University of Science and Technology “MISIS”, Leninskiy pr., 6s7, 119049 Moscow, Russia
Mikhail Kiselevskiy
Center for Biomedical Engineering, National University of Science and Technology “MISIS”, Leninskiy pr., 6s7, 119049 Moscow, Russia
Maria Kirsanova
Advanced Imaging Core Facility, Skolkovo Institute of Science and Technology, 3 Nobel Str., 121205 Moscow, Russia
Fedor Senatov
Center for Biomedical Engineering, National University of Science and Technology “MISIS”, Leninskiy pr., 6s7, 119049 Moscow, Russia
The present work aimed to study the synergistic response of bioresorbable polylactide/bioactive wollastonite scaffolds towards mechanical stability, mesenchymal stromal cell colonization, and antibacterial activity in the physiological environment. Wollastonite was synthesized at 800 °C within 2 h by sol-gel combustion method. The surface area was found to be 1.51 m2/g, and Transmission Electron Microscopy (TEM) micrographs indicated the presence of porous structures. Fused deposition modeling was used to prepare 3D-printed polylactide/wollastonite and polylactide/hydroxyapatite scaffolds. Scanning Electron Microscopy (SEM) micrographs confirmed the interconnected porous structure and complex geometry of the scaffolds. The addition of wollastonite decreased the contact angle of the scaffolds. The mechanical testing of scaffolds examined by computational simulation, as well as machine testing, revealed their non-load-bearing capacity. The chemical constituent of the scaffolds was found to influence the attachment response of different cells on their surface. The incorporation of wollastonite effectively reduced live bacterial attachment, whereas the colonization of mesenchymal cells was improved. This observation confirms polylactide/wollastonite scaffold possesses both bactericidal as well as cytocompatible properties. Thus, the risk of peri-implant bacterial film formation can be prevented, and the biological fixation of the scaffold at the defect site can be enhanced by utilizing these composites.
