Preparation, characterization, and biological activity of the inclusion complex of dihydroquercetin and β-Cyclodextrin
Yaping Xu,
Yue Wang,
Chujie Li,
Tao Han,
Haiming Chen,
Wenxue Chen,
Qiuping Zhong,
Jianfei Pei,
Guido R. M. M. Haenen,
Zhengwen Li,
Mohamed Moalin,
Ming Zhang,
Weijun Chen
Affiliations
Yaping Xu
College of Food Science and Engineering, Hainan University
Yue Wang
Department of Pharmacology and Personalized Medicine, School of Nutrition and Translational Research in Metabolism (NUTRIM); Cardiovascular Research Institute= Maastricht (CARIM), Faculty of Health, Medicine and Life Sciences, Maastricht University
Chujie Li
Department of Pharmacology and Personalized Medicine, School of Nutrition and Translational Research in Metabolism (NUTRIM); Cardiovascular Research Institute= Maastricht (CARIM), Faculty of Health, Medicine and Life Sciences, Maastricht University
Tao Han
College of Food Science and Engineering, Hainan University
Haiming Chen
College of Food Science and Engineering, Hainan University
Wenxue Chen
College of Food Science and Engineering, Hainan University
Qiuping Zhong
College of Food Science and Engineering, Hainan University
Jianfei Pei
College of Food Science and Engineering, Hainan University
Guido R. M. M. Haenen
Department of Pharmacology and Personalized Medicine, School of Nutrition and Translational Research in Metabolism (NUTRIM); Cardiovascular Research Institute= Maastricht (CARIM), Faculty of Health, Medicine and Life Sciences, Maastricht University
Zhengwen Li
School of Pharmacy, Chengdu University
Mohamed Moalin
Research Centre Material Sciences, Zuyd University of Applied Science
Ming Zhang
College of Food Science and Engineering, Hainan University
Weijun Chen
College of Food Science and Engineering, Hainan University
Abstract Dihydroquercetin (DHQ) is a natural occurring dihydroflavonol that has strong antioxidant and antibacterial activities. However, its application is limited due to its poor solubility. This study aims to improve the aqueous solubility of DHQ by complexing DHQ with β-cyclodextrin (β-CD) to boost its biological activity. DHQ was encapsulated with β-CD by freeze drying at a 1:1-M ratio. The structure of DHQ/β-CD complex prepared was elucidated by using Fourier transform infrared spectroscopy, differential scanning calorimetry, X-ray diffraction, scanning electron microscopy, and 1H nuclear magnetic resonance (1H NMR). In addition, molecular docking further revealed two energetically favorable conformations of the DHQ/β-CD complex, in which DHQ interacted with β-CD via hydrogen bonds. Experimental results showed that the solubility of the DHQ increased 22.63-fold by encapsulating with β-CD. Also the dissolution rate, antioxidant activity and antibacterial activity of the DHQ were significantly improved by encapsulating. The encapsulating with β-CD solves the problem of the poor aqueous solubility of DHQ, and broadens the path for a more optimal use of the health promoting effect of DHQ in pharmaceutical and food products.
