Comprehensive analysis of lysine lactylation in Frankliniella occidentalis

Dong An; Liyun Song; Ying Li; Lili Shen; Pu Miao; Yujie Wang; Dongyang Liu; Lianqiang Jiang; Fenglong Wang; Jinguang Yang

doi:10.3389/fgene.2022.1014225

Frontiers in Genetics (Nov 2022)

Comprehensive analysis of lysine lactylation in Frankliniella occidentalis

Dong An,
Liyun Song,
Ying Li,
Lili Shen,
Pu Miao,
Yujie Wang,
Dongyang Liu,
Lianqiang Jiang,
Fenglong Wang,
Jinguang Yang

Affiliations

Dong An: Key Laboratory of Tobacco Pest Monitoring, Controlling and Integrated Management, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, China
Liyun Song: Key Laboratory of Tobacco Pest Monitoring, Controlling and Integrated Management, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, China
Ying Li: Key Laboratory of Tobacco Pest Monitoring, Controlling and Integrated Management, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, China
Lili Shen: Key Laboratory of Tobacco Pest Monitoring, Controlling and Integrated Management, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, China
Pu Miao: Luoyang City Company of Henan Province Tobacco Company, Luoyang, China
Yujie Wang: Luoyang City Company of Henan Province Tobacco Company, Luoyang, China
Dongyang Liu: Liangshan State Company of Sichuan Province Tobacco Company, Mile, China
Lianqiang Jiang: Liangshan State Company of Sichuan Province Tobacco Company, Mile, China
Fenglong Wang: Key Laboratory of Tobacco Pest Monitoring, Controlling and Integrated Management, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, China
Jinguang Yang: Key Laboratory of Tobacco Pest Monitoring, Controlling and Integrated Management, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, China

DOI: https://doi.org/10.3389/fgene.2022.1014225
Journal volume & issue: Vol. 13

Abstract

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Western flower thrips (Frankliniella occidentalis) are among the most important pests globally that transmit destructive plant viruses and infest multiple commercial crops. Lysine lactylation (Klac) is a recently discovered novel post-translational modification (PTM). We used liquid chromatography-mass spectrometry to identify the global lactylated proteome of F. occidentalis, and further enriched the identified lactylated proteins using Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO). In the present study, we identified 1,458 Klac sites in 469 proteins from F. occidentalis. Bioinformatics analysis showed that Klac was widely distributed in F. occidentalis proteins, and these Klac modified proteins participated in multiple biological processes. GO and KEGG enrichment analysis revealed that Klac proteins were significantly enriched in multiple cellular compartments and metabolic pathways, such as the ribosome and carbon metabolism pathways. Two Klac proteins were found to be involved in the regulation of the TSWV (Tomato spotted wilt virus) transmission in F. occidentalis. This study provides a systematic report and a rich dataset of lactylation in F. occidentalis proteome for potential studies on the Klac protein of this notorious pest.

Published in Frontiers in Genetics

ISSN: 1664-8021 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Biology (General): Genetics
Website: http://journal.frontiersin.org/journal/genetics

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