About Samples in the Research of Intraspecific Genetic Diversity of Scots Pine (Pinus sylvestris L.)

Abstract

The influence of sample size on the results of the assessments of the genetic diversity of populations of Scots pine on the variability of 12 polymorphic loci isoenzymes and 4 chloroplast microsatellite loci (cpSSR). It was shown that increasing the sample size (to over 100 trees in the population) leads to significant increase in estimates of allozyme diversity (number of alleles and genotypes) and haplotype diversity of chloroplast DNA (microsatellite loci cpSSR) and to the reduction of inter-population genetic distance Nei without significantly changing the parameters characterizing the genetic structure of the species. Standard sample sizes allow reliable estimation of the population genetic structure of the species, but is not sufficient to study its intra-population diversity.

Keywords

• the sample
• genetic polymorphism
• Scots pine (Pinus sylvestris L.)