Cell Culture-Based Assessment of Toxicity and Therapeutics of Phytochemical Antioxidants
Peace C. Asuzu,
Nicholas S. Trompeter,
Carlton R. Cooper,
Samuel A. Besong,
Alberta N. A. Aryee
Affiliations
Peace C. Asuzu
Department of Human Ecology (Food Science and Biotechnology Program), College of Agriculture, Science and Technology, Delaware State University, Dover, DE 19901, USA
Nicholas S. Trompeter
Department of Biomedical Engineering, University of Delaware, Newark, DE 19716, USA
Carlton R. Cooper
Center for Translational Cancer Research, Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA
Samuel A. Besong
Department of Human Ecology (Food Science and Biotechnology Program), College of Agriculture, Science and Technology, Delaware State University, Dover, DE 19901, USA
Alberta N. A. Aryee
Department of Human Ecology (Food Science and Biotechnology Program), College of Agriculture, Science and Technology, Delaware State University, Dover, DE 19901, USA
Plant-derived natural products are significant resources for drug discovery and development including appreciable potentials in preventing and managing oxidative stress, making them promising candidates in cancer and other disease therapeutics. Their effects have been linked to phytochemicals such as phenolic compounds and their antioxidant activities. The abundance and complexity of these bio-constituents highlight the need for well-defined in vitro characterization and quantification of the plant extracts/preparations that can translate to in vivo effects and hopefully to clinical use. This review article seeks to provide relevant information about the applicability of cell-based assays in assessing anti-cytotoxicity of phytochemicals considering several traditional and current methods.
