Near-Infrared Fluorescence Imaging of Carotid Plaques in an Atherosclerotic Murine Model

Xiaotian Wu; Amy Daniel Ulumben; Steven Long; Wataru Katagiri; Moses Q. Wilks; Hushan Yuan; Brian Cortese; Chengeng Yang; Satoshi Kashiwagi; Hak Soo Choi; Marc D. Normandin; Georges El Fakhri; Raiyan T. Zaman

doi:10.3390/biom11121753

Biomolecules (Nov 2021)

Near-Infrared Fluorescence Imaging of Carotid Plaques in an Atherosclerotic Murine Model

Xiaotian Wu,
Amy Daniel Ulumben,
Steven Long,
Wataru Katagiri,
Moses Q. Wilks,
Hushan Yuan,
Brian Cortese,
Chengeng Yang,
Satoshi Kashiwagi,
Hak Soo Choi,
Marc D. Normandin,
Georges El Fakhri,
Raiyan T. Zaman

Affiliations

Xiaotian Wu: Gordon Center for Medical Imaging, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
Amy Daniel Ulumben: Gordon Center for Medical Imaging, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
Steven Long: Department of Pathology, University of California, San Francisco, CA 94143, USA
Wataru Katagiri: Gordon Center for Medical Imaging, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
Moses Q. Wilks: Gordon Center for Medical Imaging, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
Hushan Yuan: Gordon Center for Medical Imaging, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
Brian Cortese: Gordon Center for Medical Imaging, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
Chengeng Yang: Gordon Center for Medical Imaging, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
Satoshi Kashiwagi: Gordon Center for Medical Imaging, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
Hak Soo Choi: Gordon Center for Medical Imaging, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
Marc D. Normandin: Gordon Center for Medical Imaging, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
Georges El Fakhri: Gordon Center for Medical Imaging, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
Raiyan T. Zaman: Gordon Center for Medical Imaging, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA

DOI: https://doi.org/10.3390/biom11121753
Journal volume & issue: Vol. 11, no. 12
p. 1753

Abstract

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Successful imaging of atherosclerosis, one of the leading global causes of death, is crucial for diagnosis and intervention. Near-infrared fluorescence (NIRF) imaging has been widely adopted along with multimodal/hybrid imaging systems for plaque detection. We evaluate two macrophage-targeting fluorescent tracers for NIRF imaging (TLR4-ZW800-1C and Feraheme-Alexa Fluor 750) in an atherosclerotic murine cohort, where the left carotid artery (LCA) is ligated to cause stenosis, and the right carotid artery (RCA) is used as a control. Imaging performed on dissected tissues revealed that both tracers had high uptake in the diseased vessel compared to the control, which was readily visible even at short exposure times. In addition, ZW800-1C’s renal clearance ability and Feraheme’s FDA approval puts these two tracers in line with other NIRF tracers such as ICG. Continued investigation with these tracers using intravascular NIRF imaging and larger animal models is warranted for clinical translation.

Published in Biomolecules

ISSN: 2218-273X (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: https://www.mdpi.com/journal/biomolecules

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Abstract

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