Nature Communications (Mar 2022)
Mutation-specific reporter for optimization and enrichment of prime editing
- I. F. Schene,
- I. P. Joore,
- J. H. L. Baijens,
- R. Stevelink,
- G. Kok,
- S. Shehata,
- E. F. Ilcken,
- E. C. M. Nieuwenhuis,
- D. P. Bolhuis,
- R. C. M. van Rees,
- S. A. Spelier,
- H. P. J. van der Doef,
- J. M. Beekman,
- R. H. J. Houwen,
- E. E. S. Nieuwenhuis,
- S. A. Fuchs
Affiliations
- I. F. Schene
- Division of Pediatric Gastroenterology, Wilhelmina Children’s Hospital, University Medical Center Utrecht
- I. P. Joore
- Division of Pediatric Gastroenterology, Wilhelmina Children’s Hospital, University Medical Center Utrecht
- J. H. L. Baijens
- Utrecht University Graduate School of Life Sciences
- R. Stevelink
- Department of Genetics, UMC Utrecht Brain Center, University Medical Center Utrecht, Utrecht University
- G. Kok
- Division of Pediatric Gastroenterology, Wilhelmina Children’s Hospital, University Medical Center Utrecht
- S. Shehata
- Division of Pediatric Gastroenterology, Wilhelmina Children’s Hospital, University Medical Center Utrecht
- E. F. Ilcken
- Department of Metabolic Diseases, Wilhelmina Children’s Hospital, University Medical Center Utrecht
- E. C. M. Nieuwenhuis
- Department of Metabolic Diseases, Wilhelmina Children’s Hospital, University Medical Center Utrecht
- D. P. Bolhuis
- Department of Metabolic Diseases, Wilhelmina Children’s Hospital, University Medical Center Utrecht
- R. C. M. van Rees
- Department of Metabolic Diseases, Wilhelmina Children’s Hospital, University Medical Center Utrecht
- S. A. Spelier
- Regenerative Medicine Center Utrecht
- H. P. J. van der Doef
- Department of Pediatric Gastroenterology, University Medical Center Groningen
- J. M. Beekman
- Regenerative Medicine Center Utrecht
- R. H. J. Houwen
- Division of Pediatric Gastroenterology, Wilhelmina Children’s Hospital, University Medical Center Utrecht
- E. E. S. Nieuwenhuis
- Division of Pediatric Gastroenterology, Wilhelmina Children’s Hospital, University Medical Center Utrecht
- S. A. Fuchs
- Department of Metabolic Diseases, Wilhelmina Children’s Hospital, University Medical Center Utrecht
- DOI
- https://doi.org/10.1038/s41467-022-28656-3
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 10
Abstract
While prime editing is a promising technique, some genomic sites remain difficult to edit. Here the authors present fluoPEER, fluorescent prime editing and enrichment reporter, to rank the efficiency of pegRNAs and prime editor variants.
