Prophylactic impacts of Lagenaria siceraria against cardiomyopathy induced by doxorubicin in a rat model

Abstract

Background: Doxorubicin (DOX) is a chemotherapeutic drug applied clinically for the remedy of cancer, but its possibly life-threatening cardiotoxicity effects remain a concern. Aim: After that, this study evaluates the cardioprotective impacts of Lagenaria siceraria (LSS) oil on doxorubicin-induced cardiomyopathy in rats. Methods: Wistar male rats (n= 28, weighting 190-210 g) were arbitrarily allocated into four equal groups. Group 1 (CTR) received normal saline orally (1 ml/kg); group 2 (DOX) received DOX (10 mg/kg); group 3 (DOLS) received DOX + 3g of Lagenaria siceraria seeds oil/kg and group 4 (LSSO) received Lagenaria siceraria seeds oil (3 g/kg) daily for 18 days. The serum samples were collected to determine the creatine kinase-MB (CK-MB) isoenzyme, lactate dehydrogenase (LDH), aspartate aminotransferase (AST), and Troponin I activity. At the same time, the catalase, malondialdehyde (MDA), and reduced glutathione (GSH) were assessed in heart tissues. Additionally, histopathological investigations for the heart tissue were performed. Results: Results revealed no significant change in CK-MB levels between the DOLS group compared to the CTR group (p>0.05). DOX group confirmed a substantial increase in AST, LDH, and Troponin1 serum levels compared to DOLS and LLSO groups (p [Open Vet J 2024; 14(7.000): 1668-1676]

Keywords

• antioxidant activity
• lagenaria siceraria
• doxorubicin
• cardiotoxicity
• rat