Influence of natural polysaccharides on the morphology and properties of hybrid vaterite microcrystals
Nadezhda G. Balabushevich,
Liliya N. Maltseva,
Lyubov Y. Filatova,
Daniil V. Mosievich,
Pavel I. Mishin,
Margarita E. Bogomiakova,
Olga S. Lebedeva,
Marina A. Murina,
Dmitry V. Klinov,
Ekaterina A. Obraztsova,
Zaira F. Kharaeva,
Roxalana K. Firova,
Daria V. Grigorieva,
Irina V. Gorudko,
Oleg M. Panasenko,
Elena V. Mikhalchik
Affiliations
Nadezhda G. Balabushevich
Lomonosov Moscow State University, Department of Chemistry, Leninskiye Gory 1–3, 119991, Moscow, Russia
Liliya N. Maltseva
Lomonosov Moscow State University, Department of Chemistry, Leninskiye Gory 1–3, 119991, Moscow, Russia; Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Malaya Pirogovskaya st. 1a, 119435, Moscow, Russia
Lyubov Y. Filatova
Lomonosov Moscow State University, Department of Chemistry, Leninskiye Gory 1–3, 119991, Moscow, Russia
Daniil V. Mosievich
Lomonosov Moscow State University, Department of Chemistry, Leninskiye Gory 1–3, 119991, Moscow, Russia; Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Malaya Pirogovskaya st. 1a, 119435, Moscow, Russia
Pavel I. Mishin
Lomonosov Moscow State University, Department of Chemistry, Leninskiye Gory 1–3, 119991, Moscow, Russia
Margarita E. Bogomiakova
Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Malaya Pirogovskaya st. 1a, 119435, Moscow, Russia
Olga S. Lebedeva
Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Malaya Pirogovskaya st. 1a, 119435, Moscow, Russia
Marina A. Murina
Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Malaya Pirogovskaya st. 1a, 119435, Moscow, Russia
Dmitry V. Klinov
Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Malaya Pirogovskaya st. 1a, 119435, Moscow, Russia; The Peoples' Friendship University of Russia (RUDN University), Miklukho-Maklaya str. 6, 117198, Moscow, Russia
Ekaterina A. Obraztsova
Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Malaya Pirogovskaya st. 1a, 119435, Moscow, Russia
Zaira F. Kharaeva
Kabardino-Balkarian State University named after H.M. Berbekov, Faculty of Medicine, Inessa Armand st. 1a, 360004, Nalchik, Kabardino-Balkarian Republic, Russia
Roxalana K. Firova
Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Malaya Pirogovskaya st. 1a, 119435, Moscow, Russia
Daria V. Grigorieva
Belarusian State University, Nezavisimosti av. 4, 220030, Minsk, Belarus
Irina V. Gorudko
Belarusian State University, Nezavisimosti av. 4, 220030, Minsk, Belarus
Oleg M. Panasenko
Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Malaya Pirogovskaya st. 1a, 119435, Moscow, Russia; Pirogov Russian National Research Medical University, Ostrovityanova st. 1, 117997, Moscow, Russia
Elena V. Mikhalchik
Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Malaya Pirogovskaya st. 1a, 119435, Moscow, Russia; Corresponding author.
Co-precipitation of biopolymers into calcium carbonate crystals changes their physicochemical and biological properties. This work studies hybrid microcrystals of vaterite obtained in the presence of natural polysaccharides, as carriers for the delivery of proteins and enzymes. Hybrid microcrystals with dextran sulfate, chondroitin sulfate, heparin, fucoidan, and pectin were obtained and compared. The impact of polysaccharides on the morphology (particle diameter, surface area, nanocrystallite and pore size), polysaccharide content and surface charge of hybrid microcrystals was studied. Only microcrystals with fucoidan and heparin exhibited antioxidant activity against •ОН radical. The surface charge and pore size of the hybrid microcrystals affected the sorption of albumin, catalase, chymotrypsin, mucin. A decrease in the catalytic constant and Michaelis constant was observed for catalase sorbed on the hybrid crystals. The biocompatibility of microcrystals depended on the nature of the included polysaccharide: crystals with sulfated polysaccharides increased blood plasma coagulation but not platelet aggregation, and crystals with dextran sulfate had the greatest cytotoxicity against HT-29 cells but not erythrocytes. Hybrid microcrystals with all polysaccharides except chondroitin sulfate reduced erythrocyte lysis in vitro compared with vaterite crystals. The obtained results enable to create novel carriers based on hybrid vaterite crystals with polysaccharides, beneficial for the delivery of protein drugs.
