Construction of a Peptide Microarray for Auto-anti- body Detection
Vincent Cosandey,
Fabien Debrot,
Jérémy Kaeser,
Roger Marti,
Philippe Passeraub,
Jannick Pétremand,
Denis Prim,
Marc E. Pfeifer
Affiliations
Vincent Cosandey
HES-SO Valais, Institute of Life Technologies, Route du Rawyl 47, CH-1950 Sion 2, Switzerland
Fabien Debrot
Institute of Chemistry, University of Applied Sciences Western Switzerland, Freiburg, Perolles 80, CH-1705 Freiburg, Switzerland
Jérémy Kaeser
HES-SO Valais, Institute of Life Technologies, Route du Rawyl 47, CH-1950 Sion 2, Switzerland
Roger Marti
Institute of Chemistry, University of Applied Sciences Western Switzerland, Freiburg, Perolles 80, CH-1705 Freiburg, Switzerland
Philippe Passeraub
Institute of Sciences and Industrial Technologies, University of Applied Sciences Western Switzerland, Geneva, 4 rue de la Prairie, CH-1202 Geneva, Switzerland
Jannick Pétremand
HES-SO Valais, Institute of Life Technologies, Route du Rawyl 47, CH-1950 Sion 2, Switzerland
Denis Prim
HES-SO Valais, Institute of Life Technologies, Route du Rawyl 47, CH-1950 Sion 2, Switzerland
Marc E. Pfeifer
HES-SO Valais, Institute of Life Technologies, Route du Rawyl 47, CH-1950 Sion 2, Switzerland. [email protected]
Peptide and protein microarrays provide a multiplex approach to identification and quantification of protein–protein interactions (PPI), useful to study for instance antigen–antibody properties. Multivariate serology assays detecting multiple tumor auto-antibodies (TAA) is an emerging class of blood tests for cancer detection. Here we describe the efficient coupling of peptide baits derived from the BRCA1-associated RING domain protein 1 (BARD1) to a solid surface and detection of a commercially available anti-BARD1 antibody with this newly designed peptide microarray. Analytical sensitivity and specificity were shown to be comparable to a microtiter plate based enzyme-linked immunosorbent assay (ELISA).
