Effect of carvacrol on various cytokines genes expression in splenocytes of asthmatic mice

Abstract

Objective(s):With regard to pharmacological effects of carvacrol on the respiratory system, its effect on cytokines genes expression in splenocytes of asthmatic mice was examined in this study. Materials and Methods:Splenocytes were isolated from non-sensitized (control group), sensitized mice to ovalbumin (OVA) (group S), and S animals treated with dexamethasone, and three concentrations of carvacrol. IL-4, IFN-γ, TGF-β, FOXP3, and IL-17 genes expression were carried out in cultured splenocytes using the real-time PCR method. Results:Compared to the control group, IFN-γ and FOXP3 genes expression were significantly decreased (P

Keywords

• Carvacrol
• Cytokines
• Gene expression
• Real-time PCR
• Splenocyte