Established GC-FID for simultaneous determination of diterpenes and phytosterols in Plaunoi (Croton stellatopilosus Ohba)

Abstract

A gas chromatography-flame ionization detector (GC-FID) was established and validated for simultaneous determination of the diterpenes (phytol, GGOH, plaunotol) and phytosterols (campesterol, stigmasterol and -sitosterol) in plant leaves,callus and suspension cultures of Plaunoi (Croton stellatopilosus). Under the developed GC-FID conditions, the diterpenesand phytosterols were separated in a single run. The limit of quantification (LOQ) and the limit of detection (LOD) of eachstandard sample were determined. Interday and intraday precisions were less than 5%. The average recoveries of the methodwere 102.4-109.9% and all compounds showed linearity with r2 values all  0.9994. According to this newly establishedGC-FID method, the metabolites that accumulated in the leaves, green callus and suspension cultures of Plaunoi were determined. Phytol, GGOH and plaunotol were present at relatively high concentrations in plant leaves, and in considerablyreduced amounts in green callus cells. Phytol and plaunotol were not detected in suspension cell cultures.

Keywords

• Croton stellatopilosus
• diterpenes
• GC-FID
• phytosterols
• Plaunoi