PREVALENCE AND SPECIFIC REGIONAL FEATURES OF GENETIC STRUCTURE AND DRUG RESISTANCE OF TUBERCULOSIS MYCOBACTERIA OF HAARLEM FAMILY AMONG TUBERCULOSIS PATIENTS IN SARATOV REGION

Abstract

Goal: to study prevalence and specific regional features of genetic structure and drug resistance of M. tuberculosis of Haarlem family, circulating on the territory of Saratov Region.Materials and methods. Genotyping of 152 clinical isolates from sputum of active mostly new pulmonary tuberculosis patients was performed using hybridization on the biological mircochip with reagents of Spoligo-Biochip (OOO BIOCHIP-IMB, Moscow).Results. It was found out that among genetically heterogeneous strains of M. tuberculosis circulating on the territory of Saratov Region, mycobacteria of Haarlem family were registered in 23.7% of cases, i.e. practically in each fourth patient. Mycobacteria belonging to this genotype were characterized by genetically diverse structure of strains, which could be the evidence of their circulation on this territory for a continuous period of time. Mycobacteria of Haarlem family prevailed in the patients with destruction of lung tissue and positive results of sputum tests (86.1%), and multiple drug resistance was especially high in these mycobacteria (25%), which was typical of Saratov Region since according to data of other studies mycobacteria of Haarlem family were of low virulence and more associated with drug susceptible forms of tuberculosis.

Keywords

• микобактерии туберкулеза
• генотипы
• распространенность
• лекарственная устойчивость