Generation of human iPSCs from human prostate cancer-associated fibroblasts IBPi002-A

Zuzana Kahounová; Eva Slabáková; Lucia Binó; Ján Remšík; Radek Fedr; Jan Bouchal; Daniela Kurfűrstová; Radek Vrtěl; Vladimír Študent; Lucie Jurečková; Volodymyr Porokh; Aleš Hampl; Karel Souček

Stem Cell Research (Dec 2018)

Generation of human iPSCs from human prostate cancer-associated fibroblasts IBPi002-A

Zuzana Kahounová,
Eva Slabáková,
Lucia Binó,
Ján Remšík,
Radek Fedr,
Jan Bouchal,
Daniela Kurfűrstová,
Radek Vrtěl,
Vladimír Študent,
Lucie Jurečková,
Volodymyr Porokh,
Aleš Hampl,
Karel Souček

Affiliations

Zuzana Kahounová: Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic
Eva Slabáková: Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic
Lucia Binó: Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic
Ján Remšík: Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic
Radek Fedr: Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic
Jan Bouchal: Department of Clinical and Molecular Pathology, Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic
Daniela Kurfűrstová: Department of Clinical and Molecular Pathology, Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic
Radek Vrtěl: Department of Medical Genetics, University Hospital Olomouc, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic
Vladimír Študent: Department of Urology, University Hospital Olomouc, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic
Lucie Jurečková: Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
Volodymyr Porokh: Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
Aleš Hampl: Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic
Karel Souček: Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic; Corresponding author at: Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic.

Journal volume & issue: Vol. 33
pp. 255 – 259

Abstract

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A human induced pluripotent stem cell line was generated from cancer-associated fibroblasts of a 68-years old patient with diagnosed prostate adenocarcinoma (PCa). The fibroblast cell line was reprogrammed with Epi5™ Episomal iPSC Reprogramming Kit. Pluripotency of the derived transgene-free iPS cell line was confirmed both in vitro by detecting expression of factors of pluripotency on a single-cell level, and also in vivo using teratoma formation assay. This new iPS cell line may be used for differentiation into different prostate-specific cell types in differentiation studies.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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