Development of an Electrochemical Sensor Conjugated with Molecularly Imprinted Polymers for the Detection of Enrofloxacin
Amed Gallegos-Tabanico,
Jorge Jimenez-Canale,
Sergio G. Hernandez-Leon,
Alexel J. Burgara-Estrella,
Jose Carmelo Encinas-Encinas,
Jose A. Sarabia-Sainz
Affiliations
Amed Gallegos-Tabanico
Departamento de Investigación en Polímeros y Materiales, Universidad de Sonora, Boulevard Luis Encinas y Rosales s/n, Colonia Centro, Hermosillo C.P. 83000, Sonora, Mexico
Jorge Jimenez-Canale
Departamento de Investigación en Polímeros y Materiales, Universidad de Sonora, Boulevard Luis Encinas y Rosales s/n, Colonia Centro, Hermosillo C.P. 83000, Sonora, Mexico
Sergio G. Hernandez-Leon
Coordinación de Ciencia de los Alimentos, Centro de Investigación en Alimentación y Desarrollo, Hermosillo C.P. 83304, Sonora, Mexico
Alexel J. Burgara-Estrella
Departamento de Investigación en Física, Universidad de Sonora, Boulevard Luis Encinas y Rosales s/n, Colonia Centro, Hermosillo C.P. 83000, Sonora, Mexico
Jose Carmelo Encinas-Encinas
Departamento de Investigación en Polímeros y Materiales, Universidad de Sonora, Boulevard Luis Encinas y Rosales s/n, Colonia Centro, Hermosillo C.P. 83000, Sonora, Mexico
Jose A. Sarabia-Sainz
Departamento de Investigación en Física, Universidad de Sonora, Boulevard Luis Encinas y Rosales s/n, Colonia Centro, Hermosillo C.P. 83000, Sonora, Mexico
An electrochemical sensor was fabricated for the rapid and simple detection of enrofloxacin (EF). Modification of screen-printed gold electrodes (SPE) with molecularly imprinted polymers (MIPs) allowed the detection of enrofloxacin by square wave voltammetry (SWV), measuring the oxidation peak at +0.9 V. The detection principle of molecularly imprinted polymers (MIPs) is based on the formation of binding sites with affinities and specificities comparable with those of natural antibodies. The detection of enrofloxacin showed a linear range of 0.01–0.1 mM with a detection limit LOD of 0.02 mM. The development of a non-imprinted polymer (NIP) control sensor allowed for better and more efficient detection. In addition, the sensor is portable, having the advantage of analyzing and detecting molecules of interest without the need to take the sample to a laboratory.
