Immunological profile of lactate metabolism-related genes in Psoriasis a comprehensive analysis based on bulk and single-cell RNA sequencing data

Abstract

Abstract Psoriasis (Pso) is a chronic erythema squamous disease often accompanied by metabolic abnormalities. Disturbances in lactate metabolism have been observed in the skin of patients with Pso, but the role of lactate metabolism in the pathogenesis of Pso remains unclear. To identify core genes associated with lactate metabolism in Pso, we downloaded and merged two Pso-related datasets (GSE13355, GSE109248) from the GEO database. Through comprehensive analysis, we analyzed the functions associated with these hub genes and the correlation between their expression levels and immune infiltration. Additionally, we explored lactate metabolism-related hub genes in different immune cells using single-cell sequencing data. We identified four lactate metabolism-related hub genes that are highly associated with Pso. Further analysis revealed that these four hub genes were significantly correlated with the levels of immune cell infiltration. To further elucidate the impact of lactate metabolism on immune cells, we analyzed single-cell sequencing data from healthy controls and Pso patients. The expression of lactate metabolism-related hub genes was primarily observed in moDCs and T cells. These results suggest that lactate metabolism-related genes and their functions are closely associated with changes in inflammatory cells in Pso patients. This study provides new insights into the role of lactate metabolism in the pathogenesis of Pso and highlights potential therapeutic targets for the disease.

Keywords

• Lacate metabolism
• Psoriasis
• Immune infiltration
• RNA-seq
• Single cell sequencing