Relation between metallothionein, RNA/DNA and heavy metals in juveniles of colossoma macropomun (Cuvier, 1818) in natural conditions

Abstract

Metal content, Metallotionein and physiological condition (RNA/DNA ratio) were analyzed in Cachama, Colossoma macropomum from the Orinoco River. Juvenile samples were dissected and one gram of white muscle gills, kidney and liver was taken. Cu, Cd, Pb, Ni and Fe were analyzed by atomic absorption spectrophotometer using TORT and LUTSI as reference material. Metallothioneins were determined with Ellman´s reagent and RNA/DNA ratio was analyzed by fluorometric method. Results showed that Cd, Cu, Ni, Fe, and Pb levels were significantly higher in liver than muscle. There was no difference statistically between Mts concentrations in liver, kidney and gills; muscle showed the lowest Mts concentrations. Multivariate analysis ACP determined relationship between Mts-L and Cu and Mts-G and Cd. The Mts in muscle was related with Pb and Cd. There was no association between physiological condition and metals concentrations, however, the RNA/ DNA showed relationship with Mts-M and Mts-L

Keywords

• Metallotionein
• RNA/DNA ratio
• heavy metals