Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogel
Virginia Capella,
Rebeca E. Rivero,
Ana C. Liaudat,
Luis E. Ibarra,
Dardo A. Roma,
Fabrisio Alustiza,
Fernando Mañas,
César A. Barbero,
Pablo Bosch,
Claudia R. Rivarola,
Nancy Rodriguez
Affiliations
Virginia Capella
Department of Molecular Biology, National University of Río Cuarto-CONICET, National Route N° 36, 601 Km, Rio Cuarto, X5804ZAB, Argentina
Rebeca E. Rivero
Department of Chemistry, National University of Río Cuarto-CONICET, National Route N° 36, 601 Km, Rio Cuarto, X5804ZAB, Argentina
Ana C. Liaudat
Department of Molecular Biology, National University of Río Cuarto-CONICET, National Route N° 36, 601 Km, Rio Cuarto, X5804ZAB, Argentina
Luis E. Ibarra
Department of Molecular Biology, National University of Río Cuarto-CONICET, National Route N° 36, 601 Km, Rio Cuarto, X5804ZAB, Argentina
Dardo A. Roma
Department of Animal Clinic, National University of Río Cuarto, National Route N° 36, 601 Km, Rio Cuarto, X5804ZAB, Argentina
Fabrisio Alustiza
INTA Marcos Juárez, Province Route N° 12, 3 Km, Marcos Juárez, 2580, Argentina
Fernando Mañas
Department of Animal Clinic, National University of Río Cuarto, National Route N° 36, 601 Km, Rio Cuarto, X5804ZAB, Argentina
César A. Barbero
Department of Chemistry, National University of Río Cuarto-CONICET, National Route N° 36, 601 Km, Rio Cuarto, X5804ZAB, Argentina
Pablo Bosch
Department of Molecular Biology, National University of Río Cuarto-CONICET, National Route N° 36, 601 Km, Rio Cuarto, X5804ZAB, Argentina
Claudia R. Rivarola
Department of Chemistry, National University of Río Cuarto-CONICET, National Route N° 36, 601 Km, Rio Cuarto, X5804ZAB, Argentina
Nancy Rodriguez
Department of Molecular Biology, National University of Río Cuarto-CONICET, National Route N° 36, 601 Km, Rio Cuarto, X5804ZAB, Argentina; Corresponding author.
Several hydrogel surfaces present properties that simulate the mechanical and physicochemical features of extracellular matrix (ECM), providing a platform that mimic the native cellular milieus. Poly-N-isopropylacrylamide (PNIPAM) hydrogels are receiving attention in biomedical field due to their thermosensibility and soft texture. However, more extensive biocompatibility and cellular interactions studies with cell lines are needed. Therefore, the aim of this study is focus on evaluating the biocompatibility of PNIPAM through cytotoxicity, genotoxicity, and proliferation tests in murine preadipose cells (3T3-L1), human embryonic kidney cells (HEK293) and human carcinoma-derived cells (A549) in presence of hydrogel surfaces. Bioadhesive capacity above PNIPAM surfaces was also analyzed. MTT and neutral red uptake assays shown non-cytotoxic effect of PNIPAM in the studied cell lines. Genotoxicity was evaluated by the single-cell gel electrophoresis assay, where DNA damages were not detected. [3H]-thymidine staining allowed to corroborate that cell proliferation had progressed correctly. Adopted morphologies for each cell line over PNIPAM were similar to cell growing observed on polystyrene, indicating that the surfaces favor the cell attachment during 5 days' culture. The good biocompatibility of PNIPAM surfaces make it an interesting scaffold with clinical potential in tissue regeneration engineering, and a possible adipose and kidney tissue-engineered construct.
