OncoTargets and Therapy (Oct 2020)

miR-525-5p Modulates Proliferation and Epithelial–Mesenchymal Transition of Glioma by Targeting Stat-1

  • Xie P,
  • Han Q,
  • Liu D,
  • Yao D,
  • Lu X,
  • Wang Z,
  • Zuo X

Journal volume & issue
Vol. Volume 13
pp. 9957 – 9966

Abstract

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Peng Xie,1,* Qiu Han,2,* Dachao Liu,3,* Dan Yao,4 Xiaoqing Lu,5 Ziyu Wang,6 Xiaohua Zuo7 1Department of Neurosurgery, The Affiliated Huai’an Hospital of Xuzhou Medical University, The Second People’s Hospital of Huai’an, Huai’an 223002, People’s Republic of China; 2Department of Neurology, Huai’an First People’s Hospital, The Affiliated Huai’an No.1 People’s Hospital of Nanjing Medical University, Huai’an, Jiangsu 223000, People’s Republic of China; 3Department of Image, The Affiliated Huai’an Hospital of Xuzhou Medical University, The Second People’s Hospital of Huai’an, Huai’an 223002, People’s Republic of China; 4Department of General Surgery, The Affiliated Huai’an Hospital of Xuzhou Medical University, The Second People’s Hospital of Huai’an, Huai’an 223002, People’s Republic of China; 5Department of Orthopedic, The Affiliated Huai’an Hospital of Xuzhou Medical University, The Second People’s Hospital of Huai’an, Huai’an 223002, People’s Republic of China; 6Department of Emergency Intensive Care Unit, The Affiliated Huai’an Hospital of Xuzhou Medical University, The Second People’s Hospital of Huai’an, Huai’an 223002, People’s Republic of China; 7Department of Pain Management, The Affiliated Huai’an Hospital of Xuzhou Medical University, The Second People’s Hospital of Huai’an, Huai’an 223002, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xiaohua ZuoDepartment of Pain Management, The Affiliated Huai’an Hospital of Xuzhou Medical University, The Second People’s Hospital of Huai’an, No. 62, Huaihai Road (S.), Huai’an 223002, People’s Republic of ChinaEmail [email protected] WangDepartment of Emergency Intensive Care Unit, The Affiliated Huai’an Hospital of Xuzhou Medical University, The Second People’s Hospital of Huai’an, No. 62, Huaihai Road (S.), Huai’an 223002, People’s Republic of ChinaEmail [email protected]: Glioma is the most aggressive human brain tumor. Recent studies revealed that microRNAs play vital roles in glioma. However, the function of microRNA-525-5p (miR-525-5p) in glioma remains unclear.Methods: qRT-PCR and Western blotting were used to evaluate mRNA and protein levels in glioma tissues and cells. Colony formation, CCK-8, and Edu assays evaluated the growth of glioma cells. Wound-healing, transwell, and 3D invasion assays examined the migration and invasion activities of glioma cells. Luciferase reporter assays assessed the regulatory relationship interaction between miR-525-5p and Stat-1. A mouse xenograft model was used to examine the effect of miR-525-5p on glioma in vivo.Results: miR-525-5p expression was downregulated in glioma tissues and cells. Overexpressing miR-525-5p decreased the growth of glioma cells and reduced the migration, invasion, and epithelial–mesenchymal transition of glioma cells. Bioinformatics analysis identified Stat-1 as a potential target of miR-525-5p, and dual luciferase reporter assays revealed that miR-525-5p negatively regulates Stat-1. Decreased Stat-1 led to the inhibition of FOXM1, affecting NF-κB signaling activity. Overexpressing miR-525-5p reduced tumor development in vivo.Conclusion: miR-525-5p negatively regulates cell proliferation, migration, invasion, and epithelial–mesenchymal transition in glioma, and Stat 1 is a target of miR-525-5p. miR-525-5p may be a potential target for glioma treatment.Keywords: miR-525-5p, glioma, Stat 1, proliferation, EMT

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