Diagnostic Significance of FNAB miRNA Expression in Papillary Thyroid Carcinoma

Romena Laukienė; Laima Ambrozaityte; Loreta Cimbalistienė; Algirdas Utkus; Algirdas Edvardas Tamosiunas

doi:10.3390/diagnostics12061384

Diagnostics (Jun 2022)

Diagnostic Significance of FNAB miRNA Expression in Papillary Thyroid Carcinoma

Romena Laukienė,
Laima Ambrozaityte,
Loreta Cimbalistienė,
Algirdas Utkus,
Algirdas Edvardas Tamosiunas

Affiliations

Romena Laukienė: Department of Human and Medical Genetics, Institute of Biomedical Sciences, Faculty of Medicine, Vilnius University, Santariskiu 2, 08661 Vilnius, Lithuania
Laima Ambrozaityte: Department of Human and Medical Genetics, Institute of Biomedical Sciences, Faculty of Medicine, Vilnius University, Santariskiu 2, 08661 Vilnius, Lithuania
Loreta Cimbalistienė: Department of Human and Medical Genetics, Institute of Biomedical Sciences, Faculty of Medicine, Vilnius University, Santariskiu 2, 08661 Vilnius, Lithuania
Algirdas Utkus: Department of Human and Medical Genetics, Institute of Biomedical Sciences, Faculty of Medicine, Vilnius University, Santariskiu 2, 08661 Vilnius, Lithuania
Algirdas Edvardas Tamosiunas: Department of Radiology, Nuclear Medicine and Medical Physis, Institute of Biomedical Sciences, Faculty of Medicine, Vilnius University, Santariskiu 2, 08661 Vilnius, Lithuania

DOI: https://doi.org/10.3390/diagnostics12061384
Journal volume & issue: Vol. 12, no. 6
p. 1384

Abstract

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The aim of the study was to evaluate the diagnostic utility of specific miRNAs in the preoperative assessment of thyroid nodules. One hundred and sixty thyroid fine needle aspiration biopsy (FNAB) samples with suspected thyroid carcinoma were collected. To detect the levels of miRNA expression in FNAB, next generation small RNA sequencing was performed in 60 samples. Based on the results obtained, three miRNAs (miR125A, miR200B, miR4324) were selected for further analysis. Based on the most frequently reported miRNAs in the literature associated with thyroid papillary carcinoma (PTC), two more miRNA (miR146B, miR221) were selected for further validation, using real-time reverse transcriptase polymerase chain reaction (RT-PCR) in 36 benign and 64 PTC samples. Expression of miR125A, miR146B, miR221, and miR4324 was significantly higher in patients with PTC compared with benign thyroid nodules (p ˂ 0.05). miR125A and miR4324 were also significantly more highly expressed in patients with extrathyroidal tumor extension compared to those without extrathyroidal PTC extension (p p = 0.043) in patients with multifocal carcinomas compared to patients with single foci carcinomas. This prospective study showed that the expression analysis of four miRNAs (miR125A, miR146B, miR221, and miR4324) improve accuracy of FNAB, which could allow a better pre-operative diagnostic and prognostic assessment of thyroid malignancies.

Published in Diagnostics

ISSN: 2075-4418 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine: Medicine (General)
Website: http://www.mdpi.com/journal/diagnostics

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