Nature Communications (Jan 2021)

APE1 distinguishes DNA substrates in exonucleolytic cleavage by induced space-filling

  • Tung-Chang Liu,
  • Chun-Ting Lin,
  • Kai-Cheng Chang,
  • Kai-Wei Guo,
  • Shuying Wang,
  • Jhih-Wei Chu,
  • Yu-Yuan Hsiao

DOI
https://doi.org/10.1038/s41467-020-20853-2
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 12

Abstract

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The exonuclease Apurinic/apyrimidinic endonuclease 1 (APE1) is important for processing matched/mismatched terminus during DNA repair. Here the authors resolve the X-ray crystallography structures of APE1 with varied dsDNAs substrates to assay the nuclease activity.