Resistin gene expression: Novel study in dromedary camel (Camelus dromedarius)

Abstract

Resistin, an adipocyte-specific hormone involved in insulin resistance and adipocyte differentiation, was initially identified in adipose tissue and macrophages. The physiological role of this molecule in camels remains largely unexplored. This study analysed for the first time blood and tissue levels of resistin as well as expression of resistin gene by real time PCR in adipose tissue (hump, visceral & epididymal) and different muscles (gastrocnemius, heart and caecum) in dromedary camels. The re-sults revealed that resistin concentration was significantly (P<0.01) higher in epididymal adipose tissue as compared to other tissues and the lowest concentration was detected in serum. Additionally, the differential mRNA expression levels of resistin gene showed the highest expression level in epidi-dymal adipose tissue as compared to other tissues. In conclusion, the results demonstrated for the first time that resistin was expressed in different tissues of dromedary camels. These data underscore an important facet of the physiological role of resistin as a factor involved in insulin resistance and glucose metabolism in camels.

Keywords

• adipose tissue
• dromedary camel
• gene expression
• resistin