Nature Communications (May 2020)

ORF Capture-Seq as a versatile method for targeted identification of full-length isoforms

  • Gloria M. Sheynkman,
  • Katharine S. Tuttle,
  • Florent Laval,
  • Elizabeth Tseng,
  • Jason G. Underwood,
  • Liang Yu,
  • Da Dong,
  • Melissa L. Smith,
  • Robert Sebra,
  • Luc Willems,
  • Tong Hao,
  • Michael A. Calderwood,
  • David E. Hill,
  • Marc Vidal

DOI
https://doi.org/10.1038/s41467-020-16174-z
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 12

Abstract

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Most human protein-coding genes are expressed as multiple isoforms. Here the authors present ORF Capture-seq that uses cloned ORFs as probes to capture and sequence full length transcript sequences. This enables highly sensitive characterization of eukaryotic transcriptomes.