Extraction of Bioactive Compounds via Solid-State Fermentation Using <i>Aspergillus niger</i> GH1 and <i>Saccharomyces cerevisiae</i> from Pomegranate Peel
Ana L. Izábal-Carvajal,
Leonardo Sepúlveda,
Mónica L. Chávez-González,
Cristian Torres-León,
Cristóbal N. Aguilar,
Juan A. Ascacio-Valdés
Affiliations
Ana L. Izábal-Carvajal
Bioprocesses & Bioproducts Group, Food Research Department, School of Chemistry, Autonomous University of Coahuila, Saltillo 25280, Coahuila, Mexico
Leonardo Sepúlveda
Bioprocesses & Bioproducts Group, Food Research Department, School of Chemistry, Autonomous University of Coahuila, Saltillo 25280, Coahuila, Mexico
Mónica L. Chávez-González
Bioprocesses & Bioproducts Group, Food Research Department, School of Chemistry, Autonomous University of Coahuila, Saltillo 25280, Coahuila, Mexico
Cristian Torres-León
Research Center and Ethnobiological Garden (CIJE-UAdeC), Autonomous University of Coahuila, Viesca 27480, Coahuila, Mexico
Cristóbal N. Aguilar
Bioprocesses & Bioproducts Group, Food Research Department, School of Chemistry, Autonomous University of Coahuila, Saltillo 25280, Coahuila, Mexico
Juan A. Ascacio-Valdés
Bioprocesses & Bioproducts Group, Food Research Department, School of Chemistry, Autonomous University of Coahuila, Saltillo 25280, Coahuila, Mexico
This study investigated the recovery of polyphenolic compounds such as punicalagin, punicalin, and ellagic acid via solid-state fermentation (SSF)-assisted extraction from pomegranate peel (Punica granatum L.) using Aspergillus niger GH1 and Saccharomhyces cerevisiae. Food processing has contributed to the increase in agroindustrial wastes, which has become a global concern due to environmental protection. However, these wastes can be valorized via the extraction of high-value components such as bioactive compounds. Ellagitannins extracted during the bioprocesses were identified via the HPLC–MS technique and quantified via total polyphenols (hydrolyzable and condensed assays). Enzymatic activities were tested. HPLC–MS analysis showed a decrease in the levels of punicalagin, the formation of punicaline, and the accumulation of ellagic acid during fermentation kinetics. The present study compares two different bioprocesses in order to obtain, from agroindustrial wastes, high-added-value compounds using SSF-.
