Infection and Drug Resistance (Aug 2021)
Characterization of E. coli Phylogroups Causing Catheter-Associated Urinary Tract Infection
Abstract
Rasha El-Mahdy,1 Rasha Mahmoud,2 Raghdaa Shrief3 1Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt; 2Internal Medicine Department, Nephrology and Dialysis Unit, Mansoura University, Mansoura, Egypt; 3Medical Microbiology and Immunology Department, Faculty of Medicine, Damietta University, Damietta, EgyptCorrespondence: Rasha El-MahdyMedical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Mansoura, 35516, EgyptTel +20 10 0532 9819Email [email protected]: Characterization of different uropathogenic E. coli (UPEC) phylogroups is crucial to understand pathogenesis of urinary tract infection (UTI). The objective of our study was to evaluate the antibiotic resistance pattern, biofilm formation and pathogenicity islands (PAIs) of UPEC phylogroups isolated from catheter-associated UTI (CAUTI) compared to community UTI (Com-UTI).Patients and Methods: This study included 90 UPEC strains recovered from CAUTI and Com-UTI. Antimicrobial susceptibility was tested by the Kirby–Bauer method and extended spectrum beta-lactamase (ESBL) production was confirmed using the combined disk. The biofilm formation was tested using the microtiter plate assay. Main E. coli phylogroups (A, B1, B2 and D) were detected by multiplex PCR and 2 multiplex PCR detected the 8 PAIs.Results: Antibiotic resistance of UPEC strains showed a similar high resistance in CAUTI and Com-UTI. Isolates from CAUTI significantly produced biofilm higher than Com-UTI strains (68.9% vs 44.4%). In CAUTI and Com-UTI isolates, phylogroup A was the commonest (53.3% vs 48.9%, respectively). PAI IV536 was the most common in the strains from CAUTI (71.1%) and Com-UTI (73.3%). No significant relationship was detected between the studied characters and different phylogroups except the significant resistance to cefotaxime, ceftazidime and aztreonam among phylogroups from CAUTI isolates.Conclusion: Increased antibiotic resistance and ESBLs were detected in UPEC strains from CAUTI and Com-UTI. The strains from CAUTI significantly produced biofilm higher than Com-UTI strains. Phylogroup A was the predominate phylogroup and PAI IV536 was the most prevalent marker in all phylogroups from both types of UTI.Keywords: Escherichia coli, pathogenicity island, CAUTI
