Identification of methylation pattern in the partial promoter of acetyl CoA carboxylase beta (ACACB) gene in White Leghorn line

Abstract

The present study was initiated with an objective of identifying methylation pattern in the partial promoter of Acetyl CoA carboxylase beta (ACACB) gene in White Leghorn IWK line. To understand the epigenetic regulation of gene expression, birds with highest and lowest expression at 18th week and 40th week age were chosen. Epigenetic profile of the minimal promoter indicated that there were seven CpG islands in this region, positioned at 75, 87, 155, 269, 284, 311 and 426 positions of the 555 bp promoter region. Among these, except the position 75, the remaining positions showed cis-acting transcription factors. There was negative relationship between highest and lowest expressed birds with the per cent methylation in White Leghorn IWK line. The mean methylation per cent was found to be 42.85 and 21.42 at 18th and 40th weeks of age, respectively. The results indicated decrease in methylation per cent with age. The overall mean methylation per cent of 32.14 was observed.

Keywords

• ACACB gene, Methylation, Partial promoter, White Leghorn