Iraqi Journal of Pharmaceutical Sciences (Jun 2019)

The Synthesis and Preliminary Anticancer Evaluation of 6-Mercaptopurine -Methotrexate Conjugate as Possible Mutual Prodrug

  • Asmaa Safwan Al-darraji,
  • Mohamed H.Mohamed

DOI
https://doi.org/10.31351/vol28iss1pp114-124
Journal volume & issue
Vol. 28, no. 1
pp. 114 – 124

Abstract

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Small molecules drug conjugate mutual prodrug design (SMDC) composed of folate &lethal agent conjugate , rigidly bonded via hydrophilic bridge& self immolative disulfide bond ; represent new interesting approaches for cancer treatment , the component of SMDC intended to be as possible suit targeted folate receptor , along with greater conservation of component until reaching targeting tumor tissue . The designing & synthesis of compound VI & IIX derived from 6-MP & MTX conjugate altogether as mutual prodrug were processed forward successfully by multiple steps procedure , and by TLC spectrophotometry preliminary analysis assess in detection of products & their intermediate , along with their purity . The structure of two final compounds & their intermediate were proclaimed by melting point measurement , infrared spectrometry & HNMR analysis given results greatly correspond with theoretical proposed chemical structure of synthesized compounds . Furthermore anticancer evaluation on cell line level had been done for two final compound against MCF-7& SKO-3 types of cancer cell & the result were confirmed which show greater cytotoxic tumor activity of two final compound ,while compound IIX possess optimal activity proportional with increase number of 6-MP residue .

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