In recent years, nanotechnology has become indispensable in our lives, especially in the medical field. The key to nanotechnology is the perfect combination of molecular imaging and nanoscale probes. In this paper, we used iron oxide nanoparticles as a nanoprobe because it is widely used in clinical MRI and other molecular imaging techniques. We built our own experimental environment and used absorbing materials during the whole experiment to avoid electromagnetic interference with the surroundings. Moreover, we repeated the experiment many times to exclude the influence of contingency. Hence, the experimental data we obtained were relatively precise and persuasive. Finally, the results demonstrated that the iron oxide nanoparticles were appropriate for use as contrast agents in biological imaging.