Frontiers in Cell and Developmental Biology (Apr 2022)
LMNA Determines Nuclear Morphology During Syncytialization of Human Trophoblast Stem Cells
- Yiming Wang,
- Yiming Wang,
- Yiming Wang,
- Yiming Wang,
- Hao Wu,
- Hao Wu,
- Hao Wu,
- Hao Wu,
- Xiangxiang Jiang,
- Xiangxiang Jiang,
- Lei Jia,
- Meijiao Wang,
- Meijiao Wang,
- Meijiao Wang,
- Yin Rong,
- Yin Rong,
- Yin Rong,
- Yin Rong,
- Shuo Chen,
- Shuo Chen,
- Shuo Chen,
- Shuo Chen,
- Yue Wang,
- Yue Wang,
- Yue Wang,
- Yue Wang,
- Zhenyu Xiao,
- Zhenyu Xiao,
- Zhenyu Xiao,
- Zhenyu Xiao,
- Xiaoyan Liang,
- Hongmei Wang,
- Hongmei Wang,
- Hongmei Wang,
- Hongmei Wang
Affiliations
- Yiming Wang
- State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
- Yiming Wang
- University of Chinese Academy of Sciences, Beijing, China
- Yiming Wang
- Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China
- Yiming Wang
- Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, China
- Hao Wu
- State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
- Hao Wu
- University of Chinese Academy of Sciences, Beijing, China
- Hao Wu
- Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China
- Hao Wu
- Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, China
- Xiangxiang Jiang
- State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
- Xiangxiang Jiang
- NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract, Anhui Medical University, Hefei, China
- Lei Jia
- Reproductive Medical Center, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China
- Meijiao Wang
- State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
- Meijiao Wang
- Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China
- Meijiao Wang
- Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, China
- Yin Rong
- State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
- Yin Rong
- University of Chinese Academy of Sciences, Beijing, China
- Yin Rong
- Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China
- Yin Rong
- Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, China
- Shuo Chen
- State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
- Shuo Chen
- University of Chinese Academy of Sciences, Beijing, China
- Shuo Chen
- Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China
- Shuo Chen
- Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, China
- Yue Wang
- State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
- Yue Wang
- University of Chinese Academy of Sciences, Beijing, China
- Yue Wang
- Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China
- Yue Wang
- Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, China
- Zhenyu Xiao
- State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
- Zhenyu Xiao
- Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China
- Zhenyu Xiao
- Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, China
- Zhenyu Xiao
- School of Life Science, Beijing Institute of Technology, Beijing, China
- Xiaoyan Liang
- Reproductive Medical Center, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China
- Hongmei Wang
- State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
- Hongmei Wang
- University of Chinese Academy of Sciences, Beijing, China
- Hongmei Wang
- Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China
- Hongmei Wang
- Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, China
- DOI
- https://doi.org/10.3389/fcell.2022.836390
- Journal volume & issue
-
Vol. 10
Abstract
Upon implantation, the trophectoderm differentiates into the multi-nucleated primitive syncytiotrophoblast (pSTB) through a process called primary syncytialization to facilitate maternal-fetal interactions and to establish a pregnancy. However, ethical issues and limited access to human embryos around the time of embryo implantation hinder the investigation of the detailed molecular mechanisms underpinning this event in humans. Here we established human trophoblast stem cells (hTSCs) from human blastocysts. We characterized nuclear enlargement in STB differentiated from hTSCs, which recapitulate morphological nuclear features of pSTB in human embryos. Specifically, we revealed that CRISPR/Cas9-mediated LMNA disruption perturbated nuclear volume during hTSCs syncytialization. Overall, our results not only provide an interesting insight into mechanisms underlying nuclear enlargement during primary syncytialization but highlight the hTSCs as an indispensable model in understanding human trophoblast differentiation during implantation.
Keywords
