Recent Progress on the Localization of the Spindle Assembly Checkpoint Machinery to Kinetochores
Zhen Dou,
Diogjena Katerina Prifti,
Ping Gui,
Xing Liu,
Sabine Elowe,
Xuebiao Yao
Affiliations
Zhen Dou
Anhui Key Laboratory of Cellular Dynamics & Chemical Biology, Hefei National Science Center for Physical Sciences at the Microscale, School of Life Sciences, University of Science & Technology of China, Hefei 230027, China
Diogjena Katerina Prifti
Programme in Molecular and Cellular Biology, Faculty of Medicine, Université Laval, 1050 Avenue de la Medecine, Bureau 4633, Universite Laval, Quebec, QC G1V0A6, Canada
Ping Gui
Anhui Key Laboratory of Cellular Dynamics & Chemical Biology, Hefei National Science Center for Physical Sciences at the Microscale, School of Life Sciences, University of Science & Technology of China, Hefei 230027, China
Xing Liu
Anhui Key Laboratory of Cellular Dynamics & Chemical Biology, Hefei National Science Center for Physical Sciences at the Microscale, School of Life Sciences, University of Science & Technology of China, Hefei 230027, China
Sabine Elowe
Programme in Molecular and Cellular Biology, Faculty of Medicine, Université Laval, 1050 Avenue de la Medecine, Bureau 4633, Universite Laval, Quebec, QC G1V0A6, Canada
Xuebiao Yao
Anhui Key Laboratory of Cellular Dynamics & Chemical Biology, Hefei National Science Center for Physical Sciences at the Microscale, School of Life Sciences, University of Science & Technology of China, Hefei 230027, China
Faithful chromosome segregation during mitosis is crucial for maintaining genome stability. The spindle assembly checkpoint (SAC) is a surveillance mechanism that ensures accurate mitotic progression. Defective SAC signaling leads to premature sister chromatid separation and aneuploid daughter cells. Mechanistically, the SAC couples the kinetochore microtubule attachment status to the cell cycle progression machinery. In the presence of abnormal kinetochore microtubule attachments, the SAC prevents the metaphase-to-anaphase transition through a complex kinase-phosphatase signaling cascade which results in the correct balance of SAC components recruited to the kinetochore. The correct kinetochore localization of SAC proteins is a prerequisite for robust SAC signaling and, hence, accurate chromosome segregation. Here, we review recent progresses on the kinetochore recruitment of core SAC factors.
