Analysis of Five Mushroom Toxins in Blood by UPLC-HRMS
LIU Wen-qiao,
SHI Yan,
XIANG Ping,
YU Feng,
XIE Bing,
DONG Mei,
HA Jing,
MA Chun-ling,
WEN Di
Affiliations
LIU Wen-qiao
Hebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identification, Forensic Identification Center of Hebei Medical University, College of Forensic Medicine, Hebei Medical University, Shijiazhuang 050017, China
SHI Yan
Shanghai Key Laboratory of Forensic Medicine, Key Laboratory of Forensic Science, Ministry of Justice, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China
XIANG Ping
Shanghai Key Laboratory of Forensic Medicine, Key Laboratory of Forensic Science, Ministry of Justice, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China
YU Feng
Hebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identification, Forensic Identification Center of Hebei Medical University, College of Forensic Medicine, Hebei Medical University, Shijiazhuang 050017, China
XIE Bing
Hebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identification, Forensic Identification Center of Hebei Medical University, College of Forensic Medicine, Hebei Medical University, Shijiazhuang 050017, China
DONG Mei
Hebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identification, Forensic Identification Center of Hebei Medical University, College of Forensic Medicine, Hebei Medical University, Shijiazhuang 050017, China
HA Jing
College of Chemical and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China
MA Chun-ling
Hebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identification, Forensic Identification Center of Hebei Medical University, College of Forensic Medicine, Hebei Medical University, Shijiazhuang 050017, China
WEN Di
Hebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identification, Forensic Identification Center of Hebei Medical University, College of Forensic Medicine, Hebei Medical University, Shijiazhuang 050017, China
ObjectiveTo develop a method for the simultaneous and rapid detection of five mushroom toxins （α-amanitin， phallacidin， muscimol， muscarine and psilocin） in blood by ultra-high performance liquid chromatography-high resolution mass spectrometry （UPLC-HRMS）.MethodsThe blood samples were precipitated with acetonitrile-water solution（Vacetonitril∶Vwater=3∶1） and PAX powder， then separated on ACQUITY Premier C18 column， eluted gradient. Five kinds of mushroom toxins were monitored by FullMS-ddMS2/positive ion scanning mode， and qualitative and quantitative analysis was conducted according to the accurate mass numbers of primary and secondary fragment ions.ResultsAll the five mushroom toxins had good linearity in their linear range， with a determination coefficient （R2）≥0.99. The detection limit was 0.2-20 ng/mL. The ration limit was 0.5-50 ng/mL. The recoveries of low， medium and high additive levels were 89.6%-101.4%， the relative standard deviation was 1.7%-6.7%， the accuracy was 90.4%-101.3%， the intra-day precision was 0.6%-9.0%， the daytime precision was 1.7%-6.3%， and the matrix effect was 42.2%-129.8%.ConclusionThe method is simple， rapid， high recovery rate， and could be used for rapid and accurate qualitative screening and quantitative analysis of various mushroom toxins in biological samples at the same time， so as to provide basis for the identification of mushroom poisoning events.
