Effect of NAIF1 on IFIT Family Proteins in Gastric Cancer

ZHANG Qiao; HUANG Changzhi; ZHU Jianjiang; LI Yan; ZHAO Mei; LI Kexin; LI Dongdong; QI Hong; HUANG Shengkai

doi:10.3971/j.issn.1000-8578.2022.21.0551

Zhongliu Fangzhi Yanjiu (Feb 2022)

Effect of NAIF1 on IFIT Family Proteins in Gastric Cancer

ZHANG Qiao,
HUANG Changzhi,
ZHU Jianjiang,
LI Yan,
ZHAO Mei,
LI Kexin,
LI Dongdong,
QI Hong,
HUANG Shengkai

Affiliations

ZHANG Qiao: Prenatal Diagnosis Center, Beijing Haidian Maternal and Child Health Hospital, Beijing 100089, China
HUANG Changzhi: National Cancer Center/National Clinical Research Cencer for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical Colloge, Beijing 100020, China
ZHU Jianjiang: Prenatal Diagnosis Center, Beijing Haidian Maternal and Child Health Hospital, Beijing 100089, China
LI Yan: Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510000, China
ZHAO Mei: National Cancer Center/National Clinical Research Cencer for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical Colloge, Beijing 100020, China
LI Kexin: National Cancer Center/National Clinical Research Cencer for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical Colloge, Beijing 100020, China
LI Dongdong: National Cancer Center/National Clinical Research Cencer for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical Colloge, Beijing 100020, China
QI Hong: Prenatal Diagnosis Center, Beijing Haidian Maternal and Child Health Hospital, Beijing 100089, China
HUANG Shengkai: National Cancer Center/National Clinical Research Cencer for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical Colloge, Beijing 100020, China

DOI: https://doi.org/10.3971/j.issn.1000-8578.2022.21.0551
Journal volume & issue: Vol. 49, no. 2
pp. 90 – 94

Abstract

Read online

Objective To investigate the effect of NAIF1 in gastric cancer cell lines MKN45. Methods We constructed pLVX-Tight-Flag-NAIF1-puro plasmid with Tet-on system. DOX was added to induce NAIF1 expression in MKN45 cells. The cells were collected at 0, 6, 12 and 24 hours after DOX addition for gene expression microarray detection and biological analysis of differentially expressed genes. qRT-PCR and Western blot were used to verify the changes in mRNA and protein levels of the selected target differential genes. Results The biological analysis of gene microarray hybridization results showed that IFIT1, IFIT2 and IFIT3 expression significantly increased at 24h, qRT-PCR also showed this change, and Western blot further verified the change in protein level. However, IFIT5 showed no significant change in mRNA and gene expression. Conclusion Over-expression of NAIF1 in gastric cancer cells can promote the expression of some immune system-related IFIT family proteins.

Published in Zhongliu Fangzhi Yanjiu

ISSN: 1000-8578 (Print)
Publisher: Magazine House of Cancer Research on Prevention and Treatment
Country of publisher: China
LCC subjects: Medicine: Internal medicine: Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Website: http://www.zlfzyj.com

About the journal

Abstract

Keywords