Journal of Inflammation Research (Nov 2021)

Could Mesna and Celery Seed Cotherapy Modulate Oxidative Stress and Inflammation of the Urinary Bladder Induced by Ifosfamide in Rabbits?

  • Mousa AM,
  • Allemailem KS,
  • Alhumaydhi FA,
  • Alrumaihi F,
  • Almatroudi A,
  • Alwashmi AS,
  • Aljasir M,
  • Al Rugaie O,
  • Aljohani ASM,
  • Soliman KEA,
  • Rezk MY,
  • Hegazy AMS,
  • Seleem HS

Journal volume & issue
Vol. Volume 14
pp. 5837 – 5847

Abstract

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Ayman M Mousa,1,2 Khaled S Allemailem,1,3 Fahad A Alhumaydhi,3 Faris Alrumaihi,3 Ahmad Almatroudi,3 Ameen S Alwashmi,3 Mohammad Aljasir,3 Osamah Al Rugaie,4 Abdullah SM Aljohani,5 Khaled EA Soliman,4,6 Mohamad Y Rezk,4,7 Ahmed MS Hegazy,8,9 Hanan S Seleem4,10 1Department of Basic Health Sciences, College of Applied Medical Sciences, Qassim University, Buraydah, 51452, Saudi Arabia; 2Department of Histology and Cell Biology, Faculty of Medicine, Benha University, Benha, 13518, Egypt; 3Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraydah, 51452, Saudi Arabia; 4Department of Basic Medical Sciences, College of Medicine and Medical Sciences, Qassim University, Unaizah, 51452, Saudi Arabia; 5Department of Veterinary Medicine, College of Agricultural and Veterinary Medicine, Qassim University, Buraydah, 51452, Saudi Arabia; 6Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Sohag University, Sohag, 82524, Egypt; 7Department of Medical Physiology, Faculty of Medicine, Zagazig University, Al-Sharquia, 44519, Egypt; 8Department of Anatomy, Faculty of Medicine, Northern Border University, Arar, Saudi Arabia; 9Department of Anatomy and Embryology, Faculty of Medicine, Benha University, Benha, 13518, Egypt; 10Department of Histology and Cell Biology, Faculty of Medicine, Menoufia University, Shebin Elkoum, EgyptCorrespondence: Ayman M MousaDepartment of Basic Health Sciences, College of Applied Medical Sciences, Qassim University, Buraydah, Saudi ArabiaTel +966 598146171Email [email protected]: Ifosfamide (IFS) has potential complications such as nephropathy and hemorrhagic cystitis (HC). Although mesna can prevent IFS-induced cystitis by direct binding and neutralization of acrolein, HC symptoms have still been observed clinically in most of these cases. Celery is a powerful healing vegetable that has antioxidant, anti-inflammatory, and anticancer effects. The current study evaluated the synergistic effects of mesna and celery seed on IFS-induced HC in rabbits.Methods: Twenty male rabbits (four groups) were administered distilled water, IFS, mesna, and mesna+celery seed cotherapy (MCC) for three weeks. The serum and urinary bladder of experimental rabbits underwent biochemical (TNF-α, MDA, iNOS, SOD, GPx, and CAT), histopathological and ultrastructural investigations to evaluate the structural changes of the urinary bladder (UB).Results: IFS injection resulted in severe cystitis with a remarkable increase in the scale of hematuria, elevations of TNF-α, MDA, and iNOS activity, and reduced activity of SOD, GPx, and CAT antioxidants. Additionally, the structure of UB exhibited evident mucosal edema and ulceration. In contrast, the MCC regimen group revealed partial synergistic improvement of all mentioned parameters.Conclusion: IFS induced cystitis by releasing acrolein, which exerted a significant role in the pathogenesis of HC. In contrast, the MCC intake partially ameliorated the UB damage through its antioxidant and anti-inflammatory effects.Keywords: ifosfamide, mesna, celery, hemorrhagic cystitis, antioxidants, anti-inflammatory

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