Circa-SCOPE: high-throughput live single-cell imaging method for analysis of circadian clock resetting

Gal Manella; Dan Aizik; Rona Aviram; Marina Golik; Gad Asher

doi:10.1038/s41467-021-26210-1

Nature Communications (Oct 2021)

Circa-SCOPE: high-throughput live single-cell imaging method for analysis of circadian clock resetting

Gal Manella,
Dan Aizik,
Rona Aviram,
Marina Golik,
Gad Asher

Affiliations

Gal Manella: Department of Biomolecular Sciences, Weizmann Institute of Science
Dan Aizik: Department of Biomolecular Sciences, Weizmann Institute of Science
Rona Aviram: Department of Biomolecular Sciences, Weizmann Institute of Science
Marina Golik: Department of Biomolecular Sciences, Weizmann Institute of Science
Gad Asher: Department of Biomolecular Sciences, Weizmann Institute of Science

DOI: https://doi.org/10.1038/s41467-021-26210-1
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 14

Abstract

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Phase Transition Curves (PTCs) describe phase shifts of circadian oscillations due to a stimulus and they are important for studying circadian clock resetting. Here, the authors present a method for high-throughput reconstruction of PTCs using fluorescent live imaging and single-cell analysis.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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