Data for the co-expression and purification of human recombinant CaMKK2 in complex with calmodulin in Escherichia coli

Lisa Gerner; Steffi Munack; Koen Temmerman; Ann-Marie Lawrence-Dörner; Hüseyin Besir; Matthias Wilmanns; Jan Kristian Jensen; Bernd Thiede; Ian G. Mills; Jens Preben Morth

Data in Brief (Sep 2016)

Data for the co-expression and purification of human recombinant CaMKK2 in complex with calmodulin in Escherichia coli

Lisa Gerner,
Steffi Munack,
Koen Temmerman,
Ann-Marie Lawrence-Dörner,
Hüseyin Besir,
Matthias Wilmanns,
Jan Kristian Jensen,
Bernd Thiede,
Ian G. Mills,
Jens Preben Morth

Affiliations

Lisa Gerner: Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, Forskningsparken, University of Oslo and Oslo University Hospitals, 0349 Oslo, Norway
Steffi Munack: Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, Forskningsparken, University of Oslo and Oslo University Hospitals, 0349 Oslo, Norway
Koen Temmerman: European Molecular Biology Laboratory Hamburg, Notkestrasse 85, 22603 Hamburg, Germany; European Molecular Biology Laboratory Heidelberg, Meyerhofstrasse 1, 69117 Heidelberg, Germany
Ann-Marie Lawrence-Dörner: European Molecular Biology Laboratory Heidelberg, Meyerhofstrasse 1, 69117 Heidelberg, Germany; Institute of Biochemistry, WWU Münster, Wilhelm-Klemm-Straße 2, 48149 Münster, Germany
Hüseyin Besir: European Molecular Biology Laboratory Heidelberg, Meyerhofstrasse 1, 69117 Heidelberg, Germany
Matthias Wilmanns: European Molecular Biology Laboratory Hamburg, Notkestrasse 85, 22603 Hamburg, Germany
Jan Kristian Jensen: Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus, Denmark
Bernd Thiede: Department of Biosciences, University of Oslo, Norway; Biotechnology Centre of Oslo, University of Oslo, Norway
Ian G. Mills: Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, Forskningsparken, University of Oslo and Oslo University Hospitals, 0349 Oslo, Norway; Department of Molecular Oncology, Institute for Cancer Research, Oslo University Hospitals, Norway; Movember/Prostate Cancer UK Centre of Excellence for Prostate Cancer Research, Centre for Cancer Research and Cell Biology (CCRCB), Queen׳s University Belfast, UK; Corresponding authors at: Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, Forskningsparken, University of Oslo and Oslo University Hospitals, 0349 Oslo, Norway.
Jens Preben Morth: Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, Forskningsparken, University of Oslo and Oslo University Hospitals, 0349 Oslo, Norway; Institute for Experimental Medical Research, Oslo University Hospital, Norway; Corresponding authors at: Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, Forskningsparken, University of Oslo and Oslo University Hospitals, 0349 Oslo, Norway.

Journal volume & issue: Vol. 8
pp. 733 – 740

Abstract

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Calcium/calmodulin-dependent kinase kinase 2 (CaMKK2) has been implicated in a range of conditions and pathologies from prostate to hepatic cancer. Here, we describe the expression in Escherichia coli and the purification protocol for the following constructs: full-length CaMKK2 in complex with CaM, CaMKK2 ‘apo’, CaMKK2 (165-501) in complex with CaM, and the CaMKK2 F267G mutant. The protocols described have been optimized for maximum yield and purity with minimal purification steps required and the proteins subsequently used to develop a fluorescence-based assay for drug binding to the kinase, “Using the fluorescent properties of STO-609 as a tool to assist structure-function analyses of recombinant CaMKK2” [1]. Keywords: CaMKK2, Calmodulin, Fermentation

Published in Data in Brief

ISSN: 2352-3409 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Medicine (General): Computer applications to medicine. Medical informatics; Science: Science (General)
Website: http://www.journals.elsevier.com/data-in-brief/

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